Zinc in PDB 1sdx: Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites

The structure of Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites, PDB code: 1sdx was solved by T.Jabeen, S.Sharma, G.Singhal, N.Singh, T.P.Singh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.90 / 2.06
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.210, 50.570, 66.170, 90.00, 107.64, 90.00
R / Rfree (%)	19.2 / 21

The binding sites of Zinc atom in the Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites (pdb code 1sdx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites, PDB code: 1sdx:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn677 b:28.3 occ:1.00 OH A:TYR526 2.0 19.4 1.0 OH A:TYR433 2.1 16.0 1.0 OD1 A:ASP395 2.1 19.1 1.0 NE2 A:HIS595 2.3 22.7 1.0 O2 A:CO32001 2.3 23.6 1.0 O1 A:CO32001 2.3 22.6 1.0 C A:CO32001 2.6 21.8 1.0 CZ A:TYR433 3.0 14.4 1.0 CZ A:TYR526 3.1 18.7 1.0 CD2 A:HIS595 3.2 16.6 1.0 CG A:ASP395 3.2 17.4 1.0 CE1 A:HIS595 3.3 19.4 1.0 CE1 A:TYR433 3.6 16.6 1.0 CE1 A:TYR526 3.8 18.7 1.0 CB A:ASP395 3.8 17.5 1.0 O3 A:CO32001 3.9 18.6 1.0 O A:HOH3006 3.9 16.1 1.0 CE2 A:TYR433 4.0 19.6 1.0 CE2 A:TYR526 4.0 16.8 1.0 O A:HOH3007 4.0 20.4 1.0 OD2 A:ASP395 4.2 17.7 1.0 NH2 A:ARG463 4.3 28.9 1.0 CG A:HIS595 4.4 20.3 1.0 ND1 A:HIS595 4.4 20.9 1.0 CB A:THR464 4.5 19.2 1.0 CA A:ASP395 4.5 18.6 1.0 N A:ALA465 4.8 19.9 1.0 NE A:ARG463 4.8 25.8 1.0 OG1 A:THR464 4.9 17.5 1.0 N A:THR464 4.9 19.0 1.0 CD1 A:TYR433 4.9 18.6 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1002 b:39.6 occ:1.00 O A:HOH3192 2.1 49.2 1.0 OE2 A:GLU659 2.3 35.9 1.0 OE1 A:GLU659 2.4 33.0 1.0 CD A:GLU659 2.6 33.7 1.0 O A:GLY653 3.8 44.0 1.0 CG A:GLU659 4.1 30.9 1.0 O A:ARG654 4.3 34.5 1.0 CA A:PRO655 4.7 30.3 1.0 N A:THR656 4.9 27.3 1.0 C A:ARG654 4.9 36.3 1.0 CB A:GLU659 5.0 29.0 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Zinc Saturated C-Terminal Half of Bovine Lactoferrin at 2.0 A Resolution Reveals Two Additional Zinc Binding Sites within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1003 b:37.8 occ:1.00 O A:HOH3070 2.1 29.4 1.0 O A:HOH3069 2.3 46.4 1.0 OE1 A:GLU444 2.3 36.3 1.0 OE2 A:GLU444 2.6 41.0 1.0 CD A:GLU444 2.7 38.9 1.0 O A:HOH3075 3.0 37.3 1.0 O A:HOH3055 3.9 34.3 1.0 CG A:GLU444 4.2 40.3 1.0

T.Jabeen, S.Sharma, N.Singh, A.Bhushan, T.P.Singh. Structure of the Zinc-Saturated C-Terminal Lobe of Bovine Lactoferrin at 2.0 A Resolution. Acta Crystallogr.,Sect.D V. 61 1107 2005.
ISSN: ISSN 0907-4449
PubMed: 16041076
DOI: 10.1107/S0907444905016069