Zinc in PDB 8z99: Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1

Zinc Binding Sites:

The binding sites of Zinc atom in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 (pdb code 8z99). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 9 binding sites of Zinc where determined in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1, PDB code: 8z99:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Zinc binding site 1 out of 9 in 8z99

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Zinc binding site 1 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:203.3
occ:1.00
SG A:CYS81 2.3 159.8 1.0
SG A:CYS84 2.3 149.8 1.0
SG A:CYS71 2.3 161.8 1.0
SG A:CYS87 2.3 149.2 1.0
CB A:CYS71 3.0 153.6 1.0
CB A:CYS81 3.3 156.0 1.0
CB A:CYS87 3.3 143.5 1.0
N A:CYS81 3.4 152.7 1.0
N A:GLY82 3.6 146.3 1.0
CB A:CYS84 3.7 145.7 1.0
N A:CYS71 3.7 154.9 1.0
N A:CYS84 3.8 144.7 1.0
CA A:CYS81 3.8 154.7 1.0
CA A:CYS71 3.9 153.4 1.0
C A:CYS81 4.2 154.1 1.0
N A:ARG83 4.2 151.4 1.0
CA A:CYS84 4.3 144.6 1.0
C A:PRO80 4.5 144.6 1.0
CA A:CYS87 4.5 144.0 1.0
C A:VAL70 4.5 159.9 1.0
CA A:GLY82 4.6 148.2 1.0
N A:CYS87 4.6 144.5 1.0
C A:GLY82 4.8 149.8 1.0
CA A:PRO80 4.8 142.4 1.0
C A:ARG83 4.9 148.2 1.0
C A:CYS84 4.9 145.8 1.0

Zinc binding site 2 out of 9 in 8z99

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Zinc binding site 2 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn301

b:148.4
occ:1.00
SG B:CYS87 2.3 108.7 1.0
SG B:CYS81 2.3 107.7 1.0
SG B:CYS71 2.3 124.5 1.0
SG B:CYS84 2.3 102.8 1.0
N B:CYS81 3.2 94.4 1.0
CB B:CYS81 3.2 103.7 1.0
CB B:CYS71 3.3 115.7 1.0
CB B:CYS84 3.4 93.0 1.0
CB B:CYS87 3.5 88.5 1.0
N B:GLY82 3.7 99.8 1.0
CA B:CYS81 3.7 99.5 1.0
N B:CYS71 3.7 109.0 1.0
CA B:CYS71 4.1 114.6 1.0
C B:CYS81 4.2 103.6 1.0
N B:CYS84 4.2 94.8 1.0
C B:PRO80 4.3 90.4 1.0
N B:ARG83 4.4 95.9 1.0
CA B:CYS84 4.4 90.2 1.0
N B:CYS87 4.5 83.0 1.0
CA B:PRO80 4.6 89.8 1.0
CA B:CYS87 4.6 83.4 1.0
C B:VAL70 4.7 97.3 1.0
CA B:GLY82 4.7 99.5 1.0
C B:ARG83 4.7 100.8 1.0
OG1 B:THR86 4.8 90.8 1.0
CB B:PRO80 4.9 90.2 1.0
C B:GLY82 4.9 96.6 1.0

Zinc binding site 3 out of 9 in 8z99

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Zinc binding site 3 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn301

b:154.9
occ:1.00
SG C:CYS84 2.3 100.3 1.0
SG C:CYS87 2.3 89.6 1.0
SG C:CYS71 2.3 98.6 1.0
SG C:CYS81 2.3 95.7 1.0
CB C:CYS87 3.3 68.7 1.0
CB C:CYS84 3.4 79.0 1.0
CB C:CYS81 3.4 84.0 1.0
N C:CYS81 3.4 73.2 1.0
CB C:CYS71 3.6 80.8 1.0
N C:GLY82 3.7 84.8 1.0
CA C:CYS81 3.9 80.8 1.0
N C:CYS84 3.9 86.7 1.0
N C:CYS71 4.0 80.6 1.0
N C:CYS87 4.1 70.4 1.0
CA C:CYS84 4.2 80.4 1.0
OG1 C:THR86 4.2 79.4 1.0
C C:CYS81 4.3 88.2 1.0
CA C:CYS87 4.3 65.1 1.0
C C:PRO80 4.4 75.6 1.0
N C:ARG83 4.5 84.9 1.0
CA C:CYS71 4.5 80.8 1.0
CA C:PRO80 4.8 73.5 1.0
CA C:GLY82 4.8 79.0 1.0
CB C:PRO80 4.9 76.5 1.0
NH2 C:ARG56 4.9 52.0 1.0
C C:CYS84 4.9 81.6 1.0

Zinc binding site 4 out of 9 in 8z99

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Zinc binding site 4 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn301

b:163.0
occ:1.00
SG D:CYS84 2.3 103.5 1.0
SG D:CYS87 2.3 80.1 1.0
SG D:CYS81 2.3 109.5 1.0
SG D:CYS71 2.3 106.9 1.0
N D:CYS81 3.3 90.2 1.0
CB D:CYS71 3.3 98.9 1.0
CB D:CYS84 3.4 85.9 1.0
CB D:CYS81 3.4 97.1 1.0
N D:GLY82 3.4 96.4 1.0
CB D:CYS87 3.5 75.8 1.0
CA D:CYS81 3.7 92.6 1.0
N D:CYS71 4.0 94.3 1.0
C D:CYS81 4.0 95.8 1.0
N D:ARG83 4.0 108.8 1.0
N D:CYS84 4.2 92.1 1.0
C D:ARG83 4.2 111.0 1.0
CA D:CYS71 4.3 92.7 1.0
C D:PRO80 4.3 91.7 1.0
CA D:CYS84 4.4 85.2 1.0
CA D:GLY82 4.4 92.9 1.0
O D:ARG83 4.4 109.8 1.0
C D:GLY82 4.6 93.7 1.0
CA D:PRO80 4.7 86.7 1.0
CA D:CYS87 4.7 66.9 1.0
CA D:ARG83 4.8 109.7 1.0
N D:CYS87 4.9 70.0 1.0

Zinc binding site 5 out of 9 in 8z99

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Zinc binding site 5 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Zn301

b:181.4
occ:1.00
SG E:CYS84 2.3 109.5 1.0
SG E:CYS87 2.3 93.2 1.0
SG E:CYS81 2.3 121.7 1.0
SG E:CYS71 2.3 110.3 1.0
N E:CYS81 3.1 106.8 1.0
CB E:CYS81 3.3 108.7 1.0
CB E:CYS71 3.5 108.7 1.0
CB E:CYS87 3.6 84.3 1.0
CB E:CYS84 3.7 99.3 1.0
CA E:CYS81 3.7 108.3 1.0
N E:GLY82 3.9 110.7 1.0
N E:CYS71 4.0 109.7 1.0
C E:PRO80 4.1 101.0 1.0
N E:CYS84 4.1 101.2 1.0
CA E:PRO80 4.3 97.0 1.0
C E:CYS81 4.3 111.1 1.0
CA E:CYS71 4.3 109.8 1.0
CA E:CYS84 4.5 100.2 1.0
N E:ARG83 4.6 111.2 1.0
CB E:PRO80 4.7 97.2 1.0
O E:CYS71 4.7 110.7 1.0
CA E:CYS87 4.9 82.4 1.0
C E:CYS71 4.9 110.0 1.0

Zinc binding site 6 out of 9 in 8z99

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Zinc binding site 6 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn301

b:193.4
occ:1.00
SG F:CYS87 2.3 128.6 1.0
SG F:CYS71 2.3 131.2 1.0
SG F:CYS84 2.3 137.6 1.0
SG F:CYS81 2.3 146.6 1.0
CB F:CYS84 3.1 124.2 1.0
CB F:CYS71 3.4 126.9 1.0
CB F:CYS81 3.4 138.9 1.0
CB F:CYS87 3.5 118.9 1.0
N F:CYS71 3.6 126.0 1.0
N F:CYS81 3.7 139.3 1.0
N F:CYS84 3.8 126.5 1.0
CA F:CYS84 4.0 123.1 1.0
OG1 F:THR86 4.0 115.4 1.0
CA F:CYS71 4.1 126.9 1.0
CA F:CYS81 4.1 140.6 1.0
N F:GLY82 4.1 141.3 1.0
N F:CYS87 4.1 116.6 1.0
CA F:CYS87 4.5 117.2 1.0
C F:CYS84 4.6 123.8 1.0
C F:CYS81 4.6 140.2 1.0
N F:ARG83 4.6 138.8 1.0
C F:VAL70 4.6 116.0 1.0
O F:CYS84 4.8 125.0 1.0
CA F:VAL70 4.8 112.8 1.0
C F:PRO80 4.8 136.7 1.0
NH2 F:ARG56 4.9 94.4 1.0
C F:CYS71 4.9 124.9 1.0
O F:CYS71 4.9 126.4 1.0
C F:ARG83 5.0 139.1 1.0

Zinc binding site 7 out of 9 in 8z99

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Zinc binding site 7 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
H:Zn701

b:142.2
occ:1.00
CE1 H:HIS148 2.0 106.3 1.0
ND1 H:HIS64 2.3 109.1 1.0
NE2 H:HIS148 2.7 111.8 1.0
CE1 H:HIS64 3.0 106.5 1.0
OP1 N:A-15 3.1 154.5 1.0
NE2 H:HIS62 3.1 108.6 1.0
ND1 H:HIS148 3.1 105.0 1.0
CD2 H:HIS62 3.2 102.8 1.0
CG H:HIS64 3.4 107.6 1.0
CE1 H:HIS62 3.8 108.8 1.0
CB H:HIS64 3.9 104.9 1.0
CD2 H:HIS148 3.9 109.7 1.0
CG H:HIS62 4.0 95.5 1.0
NE2 H:HIS373 4.1 141.4 1.0
CG H:HIS148 4.2 105.3 1.0
P N:A-15 4.2 160.3 1.0
NE2 H:HIS64 4.2 106.3 1.0
ND1 H:HIS62 4.3 106.3 1.0
CB H:ASP169 4.4 100.2 1.0
CE1 H:HIS373 4.4 141.0 1.0
CD2 H:HIS64 4.4 106.6 1.0
O5' N:A-15 4.6 156.7 1.0
OP2 N:A-15 4.7 153.3 1.0
CB H:HIS62 4.9 92.3 1.0

Zinc binding site 8 out of 9 in 8z99

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Zinc binding site 8 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn301

b:155.9
occ:1.00
SG J:CYS87 2.3 90.6 1.0
SG J:CYS84 2.3 102.9 1.0
SG J:CYS81 2.3 93.2 1.0
SG J:CYS71 2.3 102.5 1.0
CB J:CYS84 3.2 88.5 1.0
CB J:CYS87 3.2 64.7 1.0
CB J:CYS71 3.4 105.2 1.0
CB J:CYS81 3.5 82.5 1.0
N J:CYS81 3.5 84.6 1.0
N J:GLY82 3.7 96.4 1.0
N J:CYS71 3.9 95.9 1.0
CA J:CYS81 3.9 87.9 1.0
N J:CYS84 4.0 91.3 1.0
CA J:CYS84 4.1 86.2 1.0
N J:CYS87 4.1 65.9 1.0
CA J:CYS87 4.3 60.7 1.0
CA J:CYS71 4.3 100.4 1.0
C J:CYS81 4.3 92.2 1.0
N J:ARG83 4.5 97.3 1.0
OG1 J:THR86 4.5 65.0 1.0
C J:PRO80 4.6 74.3 1.0
CA J:GLY82 4.7 90.7 1.0
C J:CYS84 4.7 85.5 1.0
NH2 J:ARG56 4.9 56.7 1.0
CA J:PRO80 4.9 70.2 1.0
C J:GLY82 4.9 92.1 1.0
C J:ARG83 4.9 101.2 1.0
C J:VAL70 5.0 84.3 1.0

Zinc binding site 9 out of 9 in 8z99

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Zinc binding site 9 out of 9 in the Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 9 of Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate- Engaged State +1 within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Zn301

b:226.7
occ:1.00
O P:CYS81 2.1 193.0 1.0
SG P:CYS87 2.3 179.2 1.0
SG P:CYS71 2.3 184.4 1.0
SG P:CYS84 2.3 187.6 1.0
CB P:CYS87 2.9 176.8 1.0
C P:CYS81 3.1 191.1 1.0
CB P:CYS84 3.2 182.1 1.0
N P:CYS81 3.2 189.8 1.0
N P:CYS84 3.7 182.6 1.0
CA P:CYS81 3.7 190.3 1.0
SG P:CYS81 3.7 188.1 1.0
CB P:CYS71 3.8 182.3 1.0
C P:PRO80 3.9 187.6 1.0
CA P:CYS84 4.0 182.3 1.0
CA P:PRO80 4.1 188.0 1.0
CA P:CYS87 4.2 177.0 1.0
N P:CYS87 4.3 176.6 1.0
N P:GLY82 4.3 181.5 1.0
CB P:CYS81 4.3 189.5 1.0
O P:CYS84 4.5 182.1 1.0
N P:ARG83 4.5 185.4 1.0
C P:CYS84 4.6 182.7 1.0
OG1 P:THR86 4.6 174.3 1.0
CB P:PRO80 4.6 188.2 1.0
N P:CYS71 4.7 181.9 1.0
CA P:GLY82 4.7 180.7 1.0
O P:PRO80 4.7 186.9 1.0
C P:GLY82 4.7 181.4 1.0
CA P:CYS71 4.8 182.7 1.0
C P:ARG83 4.9 186.1 1.0

Reference:

X.Li, Z.Deng, H.Zhang. Cryo-Em Structure of Ntr-Bound Type 7 Crispr-Cas Complex at Substrate-Engaged State +1 To Be Published.
Page generated: Thu Oct 31 14:11:11 2024

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