Zinc in PDB 8s55: Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Enzymatic activity of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
All present enzymatic activity of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure):
2.7.7.6;
3.6.4.12;
Other elements in 8s55:
The structure of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
(pdb code 8s55). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 9 binding sites of Zinc where determined in the
Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure), PDB code: 8s55:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
Zinc binding site 1 out
of 9 in 8s55
Go back to
Zinc Binding Sites List in 8s55
Zinc binding site 1 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn2001
b:205.8
occ:1.00
|
NE2
|
A:HIS84
|
2.1
|
162.6
|
1.0
|
SG
|
A:CYS81
|
2.3
|
161.1
|
1.0
|
SG
|
A:CYS71
|
2.3
|
171.1
|
1.0
|
SG
|
A:CYS74
|
2.3
|
166.5
|
1.0
|
CE1
|
A:HIS84
|
3.0
|
162.6
|
1.0
|
CD2
|
A:HIS84
|
3.0
|
162.6
|
1.0
|
CB
|
A:CYS71
|
3.0
|
171.1
|
1.0
|
CB
|
A:CYS81
|
3.5
|
161.1
|
1.0
|
ND1
|
A:HIS84
|
4.0
|
162.6
|
1.0
|
CB
|
A:CYS74
|
4.1
|
166.5
|
1.0
|
CG
|
A:HIS84
|
4.1
|
162.6
|
1.0
|
OG1
|
A:THR73
|
4.2
|
167.2
|
1.0
|
CA
|
A:CYS81
|
4.4
|
161.1
|
1.0
|
CA
|
A:GLY63
|
4.4
|
166.8
|
1.0
|
CA
|
A:CYS71
|
4.5
|
171.1
|
1.0
|
C
|
A:GLY63
|
4.5
|
166.8
|
1.0
|
CA
|
A:GLY76
|
4.6
|
170.1
|
1.0
|
O
|
A:GLY63
|
4.7
|
166.8
|
1.0
|
N
|
A:CYS74
|
4.7
|
166.5
|
1.0
|
CA
|
A:CYS74
|
4.8
|
166.5
|
1.0
|
O
|
A:CYS74
|
4.9
|
166.5
|
1.0
|
C
|
A:CYS74
|
4.9
|
166.5
|
1.0
|
O
|
A:GLN62
|
5.0
|
167.4
|
1.0
|
C
|
A:CYS81
|
5.0
|
161.1
|
1.0
|
|
Zinc binding site 2 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 2 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn2002
b:249.3
occ:1.00
|
CB
|
A:CYS114
|
2.0
|
212.9
|
1.0
|
SG
|
A:CYS114
|
2.3
|
212.9
|
1.0
|
SG
|
A:CYS184
|
2.3
|
226.1
|
1.0
|
SG
|
A:CYS111
|
2.3
|
213.0
|
1.0
|
CB
|
A:CYS184
|
2.9
|
226.1
|
1.0
|
CA
|
A:CYS114
|
3.4
|
212.9
|
1.0
|
CB
|
A:CYS111
|
3.5
|
213.0
|
1.0
|
SG
|
A:CYS154
|
3.6
|
222.4
|
1.0
|
O
|
A:PHE112
|
3.9
|
207.8
|
1.0
|
O
|
A:CYS184
|
4.0
|
226.1
|
1.0
|
N
|
A:CYS114
|
4.0
|
212.9
|
1.0
|
CB
|
A:CYS154
|
4.2
|
222.4
|
1.0
|
CA
|
A:CYS184
|
4.3
|
226.1
|
1.0
|
C
|
A:CYS114
|
4.5
|
212.9
|
1.0
|
C
|
A:CYS184
|
4.6
|
226.1
|
1.0
|
C
|
A:CYS111
|
4.6
|
213.0
|
1.0
|
C
|
A:PHE113
|
4.6
|
209.8
|
1.0
|
CA
|
A:CYS111
|
4.7
|
213.0
|
1.0
|
O
|
A:PHE113
|
4.8
|
209.8
|
1.0
|
O
|
A:GLY183
|
4.8
|
229.4
|
1.0
|
O
|
A:CYS111
|
4.8
|
213.0
|
1.0
|
C
|
A:PHE112
|
4.9
|
207.8
|
1.0
|
N
|
A:PHE112
|
5.0
|
207.8
|
1.0
|
|
Zinc binding site 3 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 3 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn1201
b:215.7
occ:1.00
|
SG
|
B:CYS1122
|
2.3
|
167.9
|
1.0
|
SG
|
B:CYS1140
|
2.3
|
181.9
|
1.0
|
SG
|
B:CYS1137
|
2.3
|
174.4
|
1.0
|
SG
|
B:CYS1119
|
2.3
|
170.3
|
1.0
|
CB
|
B:CYS1119
|
2.8
|
170.3
|
1.0
|
CB
|
B:CYS1140
|
3.5
|
181.9
|
1.0
|
CB
|
B:CYS1122
|
3.5
|
167.9
|
1.0
|
CB
|
B:CYS1137
|
3.6
|
174.4
|
1.0
|
N
|
B:CYS1122
|
3.9
|
167.9
|
1.0
|
O
|
B:CYS1122
|
4.1
|
167.9
|
1.0
|
CA
|
B:CYS1122
|
4.2
|
167.9
|
1.0
|
CA
|
B:CYS1119
|
4.3
|
170.3
|
1.0
|
C
|
B:CYS1122
|
4.4
|
167.9
|
1.0
|
N
|
B:CYS1140
|
4.6
|
181.9
|
1.0
|
CA
|
B:CYS1140
|
4.6
|
181.9
|
1.0
|
O
|
B:ARG1138
|
4.8
|
172.3
|
1.0
|
C
|
B:CYS1119
|
4.9
|
170.3
|
1.0
|
CA
|
B:CYS1137
|
4.9
|
174.4
|
1.0
|
|
Zinc binding site 4 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 4 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn301
b:210.3
occ:1.00
|
CB
|
C:CYS90
|
2.0
|
164.6
|
1.0
|
SG
|
C:CYS97
|
2.3
|
145.8
|
1.0
|
SG
|
C:CYS94
|
2.3
|
150.6
|
1.0
|
SG
|
C:CYS90
|
2.4
|
164.6
|
1.0
|
SG
|
C:CYS88
|
2.4
|
156.7
|
1.0
|
CB
|
C:CYS97
|
3.3
|
145.8
|
1.0
|
CB
|
C:CYS88
|
3.4
|
156.7
|
1.0
|
CB
|
C:CYS94
|
3.4
|
150.6
|
1.0
|
CA
|
C:CYS90
|
3.5
|
164.6
|
1.0
|
N
|
C:CYS94
|
3.7
|
150.6
|
1.0
|
CA
|
C:CYS94
|
4.0
|
150.6
|
1.0
|
N
|
C:CYS90
|
4.0
|
164.6
|
1.0
|
N
|
C:CYS97
|
4.2
|
145.8
|
1.0
|
O
|
C:CYS94
|
4.3
|
150.6
|
1.0
|
CA
|
C:CYS97
|
4.4
|
145.8
|
1.0
|
C
|
C:CYS90
|
4.4
|
164.6
|
1.0
|
C
|
C:CYS94
|
4.5
|
150.6
|
1.0
|
C
|
C:PHE93
|
4.5
|
143.8
|
1.0
|
O
|
C:CYS90
|
4.7
|
164.6
|
1.0
|
CA
|
C:CYS88
|
4.7
|
156.7
|
1.0
|
CA
|
C:PHE93
|
4.8
|
143.8
|
1.0
|
C
|
C:CYS88
|
4.9
|
156.7
|
1.0
|
C
|
C:GLU96
|
5.0
|
147.2
|
1.0
|
|
Zinc binding site 5 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 5 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn201
b:271.6
occ:1.00
|
SG
|
I:CYS42
|
2.3
|
246.7
|
1.0
|
SG
|
I:CYS39
|
2.3
|
239.1
|
1.0
|
CB
|
I:CYS42
|
2.4
|
246.7
|
1.0
|
N
|
I:CYS42
|
2.7
|
246.7
|
1.0
|
SG
|
I:CYS20
|
3.0
|
232.7
|
1.0
|
C
|
I:ASN41
|
3.0
|
235.8
|
1.0
|
CA
|
I:CYS42
|
3.1
|
246.7
|
1.0
|
CB
|
I:ASN41
|
3.3
|
235.8
|
1.0
|
O
|
I:ASN41
|
3.5
|
235.8
|
1.0
|
CA
|
I:ASN41
|
3.6
|
235.8
|
1.0
|
CB
|
I:CYS39
|
3.8
|
239.1
|
1.0
|
CB
|
I:ASN22
|
3.8
|
225.3
|
1.0
|
ND2
|
I:ASN41
|
3.9
|
235.8
|
1.0
|
CB
|
I:CYS20
|
4.0
|
232.7
|
1.0
|
N
|
I:ASN41
|
4.1
|
235.8
|
1.0
|
SG
|
I:CYS17
|
4.1
|
227.7
|
1.0
|
CG
|
I:ASN41
|
4.1
|
235.8
|
1.0
|
C
|
I:CYS42
|
4.2
|
246.7
|
1.0
|
CG
|
I:ASN22
|
4.4
|
225.3
|
1.0
|
ND2
|
I:ASN22
|
4.4
|
225.3
|
1.0
|
O
|
I:CYS42
|
4.8
|
246.7
|
1.0
|
N
|
I:ASP43
|
5.0
|
244.0
|
1.0
|
|
Zinc binding site 6 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 6 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn202
b:220.6
occ:1.00
|
SG
|
I:CYS119
|
2.3
|
183.8
|
1.0
|
SG
|
I:CYS89
|
2.3
|
182.7
|
1.0
|
SG
|
I:CYS86
|
2.3
|
181.1
|
1.0
|
SG
|
I:CYS114
|
2.3
|
175.6
|
1.0
|
CB
|
I:CYS86
|
2.9
|
181.1
|
1.0
|
CB
|
I:CYS114
|
3.1
|
175.6
|
1.0
|
CB
|
I:CYS119
|
3.3
|
183.8
|
1.0
|
O
|
I:CYS89
|
3.9
|
182.7
|
1.0
|
CB
|
I:CYS89
|
3.9
|
182.7
|
1.0
|
N
|
I:CYS89
|
4.1
|
182.7
|
1.0
|
CA
|
I:CYS86
|
4.4
|
181.1
|
1.0
|
CA
|
I:CYS89
|
4.4
|
182.7
|
1.0
|
C
|
I:CYS89
|
4.5
|
182.7
|
1.0
|
CB
|
I:HIS91
|
4.5
|
177.9
|
1.0
|
CA
|
I:CYS114
|
4.6
|
175.6
|
1.0
|
CB
|
I:ALA116
|
4.7
|
178.6
|
1.0
|
N
|
I:GLY120
|
4.7
|
174.7
|
1.0
|
CA
|
I:CYS119
|
4.7
|
183.8
|
1.0
|
C
|
I:CYS86
|
4.9
|
181.1
|
1.0
|
|
Zinc binding site 7 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 7 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 7 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn101
b:160.8
occ:1.00
|
SG
|
J:CYS44
|
2.3
|
128.5
|
1.0
|
SG
|
J:CYS45
|
2.3
|
130.3
|
1.0
|
SG
|
J:CYS10
|
2.3
|
125.9
|
1.0
|
SG
|
J:CYS7
|
2.3
|
126.1
|
1.0
|
CB
|
J:CYS7
|
3.1
|
126.1
|
1.0
|
OG1
|
J:THR9
|
3.2
|
121.4
|
1.0
|
N
|
J:CYS45
|
3.4
|
130.3
|
1.0
|
CB
|
J:CYS44
|
3.7
|
128.5
|
1.0
|
CB
|
J:CYS45
|
3.7
|
130.3
|
1.0
|
CB
|
J:CYS10
|
3.7
|
125.9
|
1.0
|
CA
|
J:CYS45
|
3.8
|
130.3
|
1.0
|
C
|
J:CYS44
|
3.9
|
128.5
|
1.0
|
NH2
|
J:ARG42
|
3.9
|
129.1
|
1.0
|
N
|
J:CYS10
|
4.0
|
125.9
|
1.0
|
CA
|
J:CYS44
|
4.4
|
128.5
|
1.0
|
CA
|
J:CYS10
|
4.4
|
125.9
|
1.0
|
N
|
J:GLY11
|
4.5
|
122.7
|
1.0
|
CA
|
J:CYS7
|
4.6
|
126.1
|
1.0
|
O
|
J:CYS44
|
4.6
|
128.5
|
1.0
|
CB
|
J:THR9
|
4.7
|
121.4
|
1.0
|
N
|
J:LYS12
|
4.9
|
125.9
|
1.0
|
CB
|
J:LYS12
|
4.9
|
125.9
|
1.0
|
N
|
J:CYS44
|
5.0
|
128.5
|
1.0
|
C
|
J:CYS7
|
5.0
|
126.1
|
1.0
|
|
Zinc binding site 8 out
of 9 in 8s55
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Zinc Binding Sites List in 8s55
Zinc binding site 8 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 8 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn101
b:215.5
occ:1.00
|
SG
|
L:CYS22
|
2.3
|
181.8
|
1.0
|
SG
|
L:CYS36
|
2.3
|
179.5
|
1.0
|
SG
|
L:CYS19
|
2.3
|
174.7
|
1.0
|
SG
|
L:CYS39
|
2.3
|
182.9
|
1.0
|
CB
|
L:CYS39
|
3.2
|
182.9
|
1.0
|
CB
|
L:CYS19
|
3.6
|
174.7
|
1.0
|
N
|
L:CYS39
|
3.6
|
182.9
|
1.0
|
CB
|
L:CYS22
|
3.7
|
181.8
|
1.0
|
CB
|
L:CYS36
|
3.8
|
179.5
|
1.0
|
CA
|
L:CYS39
|
3.9
|
182.9
|
1.0
|
N
|
L:CYS22
|
4.2
|
181.8
|
1.0
|
N
|
L:GLY40
|
4.3
|
176.1
|
1.0
|
CA
|
L:CYS22
|
4.4
|
181.8
|
1.0
|
C
|
L:CYS39
|
4.5
|
182.9
|
1.0
|
CB
|
L:GLU38
|
4.6
|
184.6
|
1.0
|
N
|
L:HIS23
|
4.7
|
171.9
|
1.0
|
C
|
L:GLU38
|
4.7
|
184.6
|
1.0
|
C
|
L:CYS22
|
4.8
|
181.8
|
1.0
|
OG1
|
L:THR24
|
4.8
|
174.4
|
1.0
|
N
|
L:TYR41
|
4.9
|
173.5
|
1.0
|
CA
|
L:CYS19
|
4.9
|
174.7
|
1.0
|
|
Zinc binding site 9 out
of 9 in 8s55
Go back to
Zinc Binding Sites List in 8s55
Zinc binding site 9 out
of 9 in the Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure)
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 9 of Rna Polymerase II Early Elongation Complex Bound to Tfiie and Tfiif - State A (Composite Structure) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
W:Zn501
b:377.4
occ:1.00
|
CB
|
W:CYS154
|
1.6
|
352.1
|
1.0
|
SG
|
W:CYS129
|
2.3
|
352.3
|
1.0
|
SG
|
W:CYS157
|
2.3
|
346.5
|
1.0
|
SG
|
W:CYS154
|
2.3
|
352.1
|
1.0
|
CB
|
W:CYS129
|
2.8
|
352.3
|
1.0
|
CE2
|
W:PHE136
|
3.1
|
344.4
|
1.0
|
CA
|
W:CYS154
|
3.1
|
352.1
|
1.0
|
N
|
W:CYS154
|
3.7
|
352.1
|
1.0
|
CZ
|
W:PHE136
|
3.8
|
344.4
|
1.0
|
CD2
|
W:PHE136
|
4.0
|
344.4
|
1.0
|
C
|
W:CYS154
|
4.0
|
352.1
|
1.0
|
SG
|
W:CYS132
|
4.1
|
349.4
|
1.0
|
CB
|
W:CYS157
|
4.1
|
346.5
|
1.0
|
CG2
|
W:THR159
|
4.2
|
343.9
|
1.0
|
CA
|
W:CYS129
|
4.3
|
352.3
|
1.0
|
O
|
W:CYS154
|
4.4
|
352.1
|
1.0
|
N
|
W:CYS157
|
4.5
|
346.5
|
1.0
|
CB
|
W:CYS132
|
4.7
|
349.4
|
1.0
|
CA
|
W:CYS157
|
4.8
|
346.5
|
1.0
|
O
|
W:THR159
|
4.9
|
343.9
|
1.0
|
N
|
W:HIS158
|
4.9
|
337.3
|
1.0
|
N
|
W:THR155
|
4.9
|
338.5
|
1.0
|
N
|
W:CYS129
|
5.0
|
352.3
|
1.0
|
C
|
W:ARG153
|
5.0
|
342.6
|
1.0
|
|
Reference:
Y.Zhan,
F.Grabbe,
E.Oberbeckmann,
C.Dienemann,
P.Cramer.
Three-Step Mechanism of Promoter Escape By Rna Polymerase II To Be Published.
Page generated: Thu Oct 31 10:48:37 2024
|