Zinc in PDB 8osx: Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp

All present enzymatic activity of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp:
2.1.1.57;

The structure of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp, PDB code: 8osx was solved by V.Kremling, J.Sprenger, D.Oberthuer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.14 / 1.83
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	168.02, 168.02, 51.7, 90, 90, 120
R / Rfree (%)	19.7 / 22.3

The structure of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp (pdb code 8osx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp, PDB code: 8osx:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4401 b:41.0 occ:1.00 NE2 B:HIS4336 2.1 48.6 1.0 SG B:CYS4327 2.3 39.5 1.0 SG B:CYS4343 2.3 40.6 1.0 SG B:CYS4330 2.3 38.7 1.0 CE1 B:HIS4336 3.0 43.6 1.0 CB B:CYS4327 3.1 39.5 1.0 CD2 B:HIS4336 3.1 47.3 1.0 CB B:CYS4343 3.3 45.2 1.0 CB B:CYS4330 3.4 37.5 1.0 N B:CYS4330 3.5 38.0 1.0 CA B:CYS4330 3.9 36.2 1.0 ND1 B:HIS4336 4.1 43.3 1.0 CG B:HIS4336 4.2 57.2 1.0 CA B:CYS4343 4.4 52.8 1.0 O2 B:EDO4404 4.5 58.0 1.0 CA B:CYS4327 4.5 38.0 1.0 CB B:TYR4329 4.5 40.1 1.0 C B:TYR4329 4.6 45.0 1.0 CB B:LYS4346 4.8 39.1 1.0 N B:LYS4346 4.8 34.2 1.0 C2 B:EDO4404 4.9 67.0 1.0 C B:CYS4327 4.9 41.9 1.0 CA B:TYR4329 4.9 42.6 1.0 O B:CYS4327 5.0 37.2 1.0 N B:TYR4329 5.0 35.6 1.0

Zinc binding site 2 out of 2 in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Atp within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4402 b:62.3 occ:1.00 SG B:CYS4381 2.3 67.3 1.0 SG B:CYS4370 2.3 63.5 1.0 SG B:CYS4383 2.4 78.0 1.0 SG B:CYS4373 2.4 58.7 1.0 CB B:CYS4370 3.0 59.6 1.0 CB B:CYS4383 3.3 81.2 1.0 CB B:CYS4381 3.4 62.5 1.0 CB B:CYS4373 3.5 58.7 1.0 O B:HOH4541 3.8 62.5 1.0 N B:CYS4373 3.8 58.2 1.0 N B:CYS4383 3.9 82.2 1.0 CA B:CYS4381 4.0 62.4 1.0 CA B:CYS4383 4.3 92.4 1.0 CA B:CYS4373 4.3 59.4 1.0 N B:SER4382 4.4 67.0 1.0 CA B:CYS4370 4.5 60.9 1.0 C B:CYS4381 4.5 63.8 1.0 CB B:VAL4372 4.6 69.7 1.0 NZ B:LYS4377 4.8 81.7 1.0 CE B:LYS4377 4.8 77.2 1.0 C B:VAL4372 4.9 60.8 1.0 C B:CYS4370 4.9 56.1 1.0 N B:VAL4372 5.0 63.7 1.0

V.Kremling, J.Sprenger, D.Oberthuer, O.Yefanov, M.Galchenkova, P.Middendorf, S.Falke, Y.Garcia, C.Ehrt, A.Kiene, B.Klopprogge, H.Chapman. Crystal Structures of Tubercidin and Adenosine Bound to the Active Site of the Sars-Cov-2 Methyltransferase NSP10-16 To Be Published.