Zinc in PDB 8j7w: Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation

The binding sites of Zinc atom in the Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation (pdb code 8j7w). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation, PDB code: 8j7w:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:253.8 occ:1.00 OD2 A:ASP74 2.0 220.2 1.0 OD2 A:ASP244 2.0 226.6 1.0 NE2 A:HIS240 2.0 209.3 1.0 CG A:ASP74 2.5 219.9 1.0 CB A:ASP74 2.7 219.2 1.0 CE1 A:HIS240 2.9 208.3 1.0 CD2 A:HIS240 2.9 206.4 1.0 CG A:ASP244 3.2 226.2 1.0 OD1 A:ASP74 3.5 219.4 1.0 CA A:ASP74 3.7 220.2 1.0 ND1 A:HIS240 4.0 206.8 1.0 CG A:HIS240 4.0 204.8 1.0 CB A:ASP244 4.0 225.5 1.0 OD1 A:ASP244 4.1 227.2 1.0 C A:ASP74 4.2 218.9 1.0 O A:ASP74 4.3 218.7 1.0 O A:HIS240 4.7 206.5 1.0 N A:ASP74 5.0 220.3 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of HZNT7-Fab Complex in Zinc State 2, Determined in Heterogeneous Conformations- One Subunit in An Inward-Facing Zinc- Bound and the Other in An Outward-Facing Zinc-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:257.4 occ:1.00 OD1 B:ASP244 1.9 217.0 1.0 OD2 B:ASP244 2.0 219.1 1.0 OD2 B:ASP74 2.0 206.2 1.0 NE2 B:HIS240 2.1 207.9 1.0 NE2 B:HIS70 2.1 215.4 1.0 CG B:ASP244 2.2 217.3 1.0 CG B:ASP74 2.7 205.5 1.0 OD1 B:ASP74 2.8 205.6 1.0 CE1 B:HIS240 2.9 205.8 1.0 CD2 B:HIS70 3.0 212.4 1.0 CD2 B:HIS240 3.1 207.3 1.0 CE1 B:HIS70 3.1 215.6 1.0 CB B:ASP244 3.6 216.0 1.0 ND1 B:HIS240 4.0 203.3 1.0 CB B:ASP74 4.1 205.1 1.0 CG B:HIS240 4.1 205.2 1.0 CG B:HIS70 4.1 211.1 1.0 ND1 B:HIS70 4.2 213.2 1.0 CA B:ASP244 4.4 216.5 1.0 N B:ASP244 4.7 216.3 1.0 OG B:SER281 4.9 165.3 1.0 O B:HIS70 4.9 211.2 1.0

H.B.Bui, S.Watanabe, N.Nomura, K.Liu, T.Uemura, M.Inoue, A.Tsutsumi, H.Fujita, K.Kinoshita, Y.Kato, S.Iwata, M.Kikkawa, K.Inaba. Cryo-Em Structures of Human Zinc Transporter ZNT7 Reveal the Mechanism of Zn 2+ Uptake Into the Golgi Apparatus. Nat Commun V. 14 4770 2023.
ISSN: ESSN 2041-1723
PubMed: 37553324
DOI: 10.1038/S41467-023-40521-5