Zinc in PDB 8hkc: Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex

All present enzymatic activity of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex:
2.7.7.6;

The structure of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex (pdb code 8hkc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex, PDB code: 8hkc:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn1501 b:94.7 occ:1.00 SG F:CYS72 2.3 68.3 1.0 SG F:CYS85 2.3 68.6 1.0 SG F:CYS88 2.3 63.2 1.0 SG F:CYS70 2.3 61.0 1.0 CB F:CYS85 3.0 68.6 1.0 CB F:CYS70 3.1 61.0 1.0 CB F:CYS88 3.6 63.2 1.0 N F:CYS72 3.8 68.3 1.0 CB F:CYS72 3.8 68.3 1.0 N F:CYS88 4.1 63.2 1.0 N F:GLY73 4.1 73.7 1.0 N F:LEU71 4.2 58.7 1.0 N F:LYS74 4.2 77.2 1.0 CA F:CYS72 4.3 68.3 1.0 CA F:CYS70 4.3 61.0 1.0 CB F:LYS74 4.3 77.2 1.0 C F:CYS70 4.4 61.0 1.0 CA F:CYS88 4.4 63.2 1.0 CA F:CYS85 4.5 68.6 1.0 C F:CYS72 4.7 68.3 1.0 C F:LEU71 4.8 58.7 1.0 CD2 F:TYR75 4.9 73.2 1.0 CD F:LYS74 4.9 77.2 1.0 CA F:LYS74 4.9 77.2 1.0 CG F:LYS74 4.9 77.2 1.0 CB F:LYS87 4.9 70.5 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of E. Coli Rnap SIGMA32 Complex within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn1502 b:62.0 occ:1.00 SG F:CYS898 2.3 38.5 1.0 SG F:CYS895 2.3 39.2 1.0 SG F:CYS814 2.3 54.5 1.0 SG F:CYS888 2.3 46.8 1.0 CB F:CYS898 3.0 38.5 1.0 CB F:CYS895 3.4 39.2 1.0 CB F:CYS814 3.4 54.5 1.0 CB F:CYS888 3.5 46.8 1.0 NH2 F:ARG883 3.7 46.6 1.0 CA F:CYS888 3.8 46.8 1.0 N F:CYS898 4.2 38.5 1.0 N F:CYS895 4.2 39.2 1.0 CA F:CYS898 4.2 38.5 1.0 N F:ASP889 4.3 52.8 1.0 CA F:CYS895 4.3 39.2 1.0 N F:CYS814 4.4 54.5 1.0 CA F:CYS814 4.5 54.5 1.0 OG1 F:THR890 4.6 50.5 1.0 CZ F:ARG883 4.6 46.6 1.0 CG2 F:THR816 4.6 64.2 1.0 C F:CYS888 4.6 46.8 1.0 O F:CYS895 4.7 39.2 1.0 C F:CYS895 4.8 39.2 1.0 NE F:ARG883 4.8 46.6 1.0 N F:CYS888 5.0 46.8 1.0

Q.Lu, T.Chen, J.Wang, F.Wang, W.Ye, L.Ma, S.Wu. Structural Insight Into the Mechanism of Sigma 32-Mediated Transcription Initiation of Bacterial Rna Polymerase Biomolecules V. 13 2023.
ISSN: ESSN 2218-273X
DOI: 10.3390/BIOM13050738