Zinc in PDB 8gs2: Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex

The binding sites of Zinc atom in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex (pdb code 8gs2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex, PDB code: 8gs2:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1702 b:99.8 occ:1.00 SG A:CYS126 2.3 61.0 1.0 SG A:CYS123 2.3 59.1 1.0 SG A:CYS115 2.3 64.0 1.0 CB A:CYS86 2.4 61.4 1.0 SG A:CYS86 3.2 71.5 1.0 CB A:CYS115 3.5 65.5 1.0 N A:CYS115 3.6 65.7 1.0 CA A:CYS86 3.6 59.2 1.0 CB A:CYS123 3.6 55.0 1.0 N A:CYS86 3.8 60.4 1.0 CB A:CYS126 3.9 52.3 1.0 C A:CYS86 4.0 61.4 1.0 CA A:CYS115 4.1 65.7 1.0 O A:CYS86 4.2 66.5 1.0 N A:GLY88 4.3 54.5 1.0 N A:CYS123 4.5 60.0 1.0 C A:PRO114 4.5 62.0 1.0 CA A:GLY88 4.6 50.2 1.0 CA A:PRO114 4.6 61.2 1.0 CA A:CYS123 4.7 57.5 1.0 N A:CYS126 4.8 53.5 1.0 N A:PRO87 4.8 53.5 1.0 NH1 A:ARG131 4.9 58.0 1.0 CA A:CYS126 4.9 48.9 1.0

Zinc binding site 2 out of 4 in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1703 b:76.7 occ:1.00 SG A:CYS477 2.3 34.2 1.0 SG A:CYS463 2.3 42.1 1.0 SG A:CYS474 2.3 38.9 1.0 SG A:CYS472 2.3 40.4 1.0 CB A:CYS477 3.2 17.2 1.0 CB A:CYS472 3.3 28.4 1.0 CB A:CYS474 3.4 21.4 1.0 CB A:CYS463 3.6 32.5 1.0 N A:CYS477 4.1 18.9 1.0 N A:CYS474 4.2 27.1 1.0 CA A:CYS477 4.2 15.2 1.0 CA A:CYS474 4.3 19.1 1.0 CA A:CYS472 4.6 31.2 1.0 CB A:ALA465 4.6 29.2 1.0 CA A:CYS463 4.7 33.9 1.0 C A:CYS472 4.7 32.1 1.0 O A:CYS474 4.7 29.2 1.0 C A:CYS474 4.8 21.4 1.0 NH1 A:ARG452 4.9 20.1 1.0 O A:CYS472 5.0 34.9 1.0

Zinc binding site 3 out of 4 in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1704 b:71.7 occ:1.00 ND1 A:HIS703 2.1 35.2 1.0 SG A:CYS708 2.3 31.2 1.0 SG A:CYS706 2.3 45.8 1.0 SG A:CYS711 2.3 38.0 1.0 CE1 A:HIS703 2.6 32.4 1.0 CG A:HIS703 3.3 31.3 1.0 CB A:CYS708 3.4 33.4 1.0 CB A:CYS706 3.5 40.2 1.0 CB A:CYS711 3.6 17.4 1.0 NE2 A:HIS703 3.9 31.4 1.0 N A:CYS708 3.9 32.8 1.0 CB A:HIS703 4.0 30.4 1.0 N A:CYS711 4.0 26.1 1.0 CA A:CYS708 4.2 31.2 1.0 CD1 A:LEU701 4.2 26.1 1.0 CA A:HIS703 4.2 29.9 1.0 CD2 A:HIS703 4.2 31.5 1.0 CA A:CYS711 4.4 23.0 1.0 O A:CYS708 4.6 36.7 1.0 C A:CYS708 4.6 33.7 1.0 CA A:CYS706 4.7 42.9 1.0 C A:CYS706 4.7 41.8 1.0 CB A:LEU710 4.8 17.3 1.0 N A:GLU707 4.8 35.0 1.0

Zinc binding site 4 out of 4 in the Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of the CAS7-11-CSX29-Guide Rna-Target Rna (Non-Matching Pfs) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1705 b:62.2 occ:1.00 SG A:CYS1342 2.3 28.1 1.0 SG A:CYS965 2.3 18.0 1.0 SG A:CYS1312 2.3 20.5 1.0 SG A:CYS1345 2.3 30.7 1.0 CB A:CYS1345 3.3 0.0 1.0 CB A:CYS1342 3.4 9.0 1.0 CB A:CYS965 3.5 8.3 1.0 CB A:CYS1312 3.6 11.4 1.0 N A:CYS1342 3.9 12.9 1.0 N A:CYS1312 3.9 18.8 1.0 N A:CYS1345 4.2 11.1 1.0 CA A:CYS1342 4.2 11.5 1.0 CA A:CYS1345 4.3 2.7 1.0 N A:CYS965 4.4 15.5 1.0 NH1 A:ARG876 4.4 21.8 1.0 CA A:CYS1312 4.4 8.7 1.0 CA A:CYS965 4.5 5.4 1.0 CG A:ARG967 4.7 11.2 1.0 C A:CYS1342 4.9 14.0 1.0 O A:CYS1342 4.9 16.3 1.0 O A:CYS965 5.0 20.5 1.0 C A:PRO1311 5.0 23.6 1.0

K.Kato, S.Okazaki, C.Schmitt-Ulms, K.Jiang, W.Zhou, J.Ishikawa, Y.Isayama, S.Adachi, T.Nishizawa, K.S.Makarova, E.V.Koonin, O.O.Abudayyeh, J.S.Gootenberg, H.Nishimasu. Rna-Triggered Protein Cleavage and Cell Growth Arrest By the Type III-E Crispr Nuclease-Protease. Science V. 378 882 2022.
ISSN: ESSN 1095-9203
PubMed: 36423304
DOI: 10.1126/SCIENCE.ADD7347