Zinc in PDB 8ftv: Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid

The structure of Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid, PDB code: 8ftv was solved by K.Kuzelka, S.K.Nair, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 2.04
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	67.626, 67.626, 185.352, 90, 90, 90
R / Rfree (%)	20.7 / 25.6

The binding sites of Zinc atom in the Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid (pdb code 8ftv). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid, PDB code: 8ftv:

Zinc binding site 1 out of 1 in the Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sgvm Methyltransferase Triple Variant (M144V/F329V/T331A) with Sah and 2-Oxo-4-Phenylbutanoic Acid within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:64.3 occ:1.00 O11 A:Y9E403 2.2 49.8 1.0 NE2 A:HIS244 2.2 52.0 1.0 NE2 A:HIS296 2.2 42.8 1.0 O A:HOH560 2.2 47.6 1.0 O12 A:Y9E403 2.4 53.2 1.0 C9 A:Y9E403 2.8 53.4 1.0 C10 A:Y9E403 3.0 50.1 1.0 CE1 A:HIS244 3.0 50.2 1.0 CD2 A:HIS296 3.2 43.8 1.0 CE1 A:HIS296 3.2 42.6 1.0 CD2 A:HIS244 3.3 46.6 1.0 O A:PHE241 4.0 45.4 1.0 OD2 A:ASP245 4.0 72.1 1.0 O13 A:Y9E403 4.1 51.1 1.0 ND1 A:HIS244 4.2 48.5 1.0 C8 A:Y9E403 4.2 55.0 1.0 OD1 A:ASP245 4.2 67.8 1.0 ND1 A:HIS296 4.3 44.0 1.0 CG A:HIS244 4.3 49.4 1.0 CG A:HIS296 4.3 44.8 1.0 CE A:MET300 4.4 47.3 1.0 CG A:ASP245 4.5 69.4 1.0 SD A:MET300 4.6 49.6 1.0 NH2 A:ARG132 4.8 38.9 1.0 C A:PHE241 4.9 54.6 1.0 O A:HOH506 4.9 56.7 1.0

S.Ju, K.P.Kuzelka, R.Guo, B.Krohn-Hansen, J.Wu, S.K.Nair, Y.Yang. A Biocatalytic Platform For Asymmetric Alkylation of Alpha-Keto Acids By Mining and Engineering of Methyltransferases Nat Commun V. 14 5704 2023.
ISSN: ESSN 2041-1723
PubMed: 37709735
DOI: 10.1038/S41467-023-40980-W