Zinc in PDB 8fix: Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch

All present enzymatic activity of Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch:
2.7.7.6;

The structure of Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch (pdb code 8fix). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch, PDB code: 8fix:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1501 b:226.1 occ:1.00 SG D:CYS85 2.3 144.5 1.0 SG D:CYS88 2.3 142.4 1.0 SG D:CYS72 2.4 152.5 1.0 CB D:CYS70 2.6 148.1 1.0 CB D:CYS85 3.1 144.5 1.0 SG D:CYS70 3.1 148.1 1.0 CA D:CYS70 3.8 148.1 1.0 CB D:CYS88 3.9 142.4 1.0 CB D:CYS72 4.1 152.5 1.0 N D:CYS72 4.1 152.5 1.0 N D:LEU71 4.1 147.0 1.0 CB D:LYS74 4.1 145.6 1.0 C D:CYS70 4.3 148.1 1.0 N D:CYS88 4.3 142.4 1.0 N D:GLY73 4.4 144.4 1.0 CE D:LYS74 4.5 145.6 1.0 CA D:CYS85 4.5 144.5 1.0 CA D:CYS72 4.6 152.5 1.0 CA D:CYS88 4.7 142.4 1.0 N D:LYS74 4.7 145.6 1.0 C D:CYS72 4.8 152.5 1.0 CD D:LYS74 4.8 145.6 1.0 C D:GLY73 5.0 144.4 1.0 N D:LYS87 5.0 143.8 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of E. Coli Rna Polymerase Backtracked Elongation Complex Harboring A Terminal Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:170.4 occ:1.00 SG D:CYS895 2.3 64.6 1.0 SG D:CYS814 2.3 89.1 1.0 SG D:CYS898 2.3 65.6 1.0 SG D:CYS888 2.3 79.1 1.0 CB D:CYS888 3.1 79.1 1.0 CB D:CYS898 3.2 65.6 1.0 CB D:CYS814 3.3 89.1 1.0 CB D:CYS895 3.6 64.6 1.0 CA D:CYS888 3.7 79.1 1.0 NH2 D:ARG883 3.8 86.5 1.0 OG1 D:THR890 3.9 73.4 1.0 N D:CYS814 4.1 89.1 1.0 N D:CYS898 4.2 65.6 1.0 CZ D:ARG883 4.2 86.5 1.0 N D:CYS895 4.3 64.6 1.0 CA D:CYS898 4.3 65.6 1.0 OG1 D:THR816 4.3 94.2 1.0 N D:ASP889 4.3 80.4 1.0 CA D:CYS814 4.3 89.1 1.0 CA D:CYS895 4.4 64.6 1.0 C D:CYS888 4.5 79.1 1.0 NE D:ARG883 4.5 86.5 1.0 O D:CYS895 4.7 64.6 1.0 NH1 D:ARG883 4.8 86.5 1.0 C D:CYS895 4.8 64.6 1.0 N D:CYS888 4.9 79.1 1.0

L.M.Wee, A.B.Tong, A.J.Florez Ariza, C.Canari-Chumpitaz, P.Grob, E.Nogales, C.J.Bustamante. A Trailing Ribosome Speeds Up Rna Polymerase at the Expense of Transcript Fidelity Via Force and Allostery. Cell V. 186 1244 2023.
ISSN: ISSN 1097-4172
PubMed: 36931247
DOI: 10.1016/J.CELL.2023.02.008