Zinc in PDB 8ej3: M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp

All present enzymatic activity of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp:
2.7.7.6;

The structure of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp (pdb code 8ej3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp, PDB code: 8ej3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2003 b:102.7 occ:1.00 SG D:CYS978 2.3 62.2 1.0 SG D:CYS891 2.3 75.6 1.0 SG D:CYS975 2.3 56.9 1.0 SG D:CYS968 2.3 66.5 1.0 CB D:CYS978 3.1 51.3 1.0 CB D:CYS975 3.5 47.8 1.0 CB D:CYS968 3.6 56.6 1.0 CB D:CYS891 3.6 68.6 1.0 NH2 D:ARG963 3.7 61.7 1.0 CA D:CYS968 4.0 61.1 1.0 N D:CYS978 4.2 51.4 1.0 CA D:CYS978 4.2 48.8 1.0 N D:ALA969 4.3 61.6 1.0 N D:CYS891 4.3 74.0 1.0 N D:CYS975 4.4 40.5 1.0 CA D:CYS975 4.5 38.0 1.0 CA D:CYS891 4.6 68.7 1.0 C D:CYS968 4.7 64.9 1.0 CZ D:ARG963 4.7 60.5 1.0 O D:CYS975 4.9 52.8 1.0 C D:CYS975 4.9 48.5 1.0

Zinc binding site 2 out of 2 in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2004 b:174.5 occ:1.00 SG D:CYS62 2.3 151.1 1.0 SG D:CYS75 2.3 146.1 1.0 SG D:CYS78 2.3 145.6 1.0 CB D:CYS75 3.0 145.4 1.0 CB D:CYS78 3.2 142.4 1.0 CB D:CYS62 3.2 149.8 1.0 N D:CYS62 3.4 150.9 1.0 CB D:CYS60 3.5 136.3 1.0 SG D:CYS60 3.7 138.4 1.0 N D:CYS78 3.7 143.4 1.0 CA D:CYS62 3.9 150.3 1.0 N D:TYR61 4.0 132.9 1.0 CA D:CYS78 4.1 142.0 1.0 C D:TYR61 4.5 133.8 1.0 CA D:CYS75 4.5 145.9 1.0 CA D:CYS60 4.6 136.4 1.0 N D:GLY63 4.6 160.9 1.0 CA D:TYR61 4.7 131.0 1.0 C D:CYS60 4.7 137.8 1.0 N D:GLY79 4.8 133.3 1.0 CB D:TYR61 4.8 129.9 1.0 C D:CYS62 4.9 150.5 1.0 C D:ARG77 4.9 146.7 1.0 CB D:ARG77 4.9 146.3 1.0 C D:CYS78 4.9 141.8 1.0

R.K.Vishwakarma, M.Z.Qayyum, P.Babitzke, K.S.Murakami. Structural Basis of Nusg-Dependent Rna Polymerase Transcription Pausing in Bacteria To Be Published.