Zinc in PDB 8btl: Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4

The structure of Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4, PDB code: 8btl was solved by S.Virdee, P.D.Mabbitt, L.Barnsby-Greer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	65.83 / 3.20
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.157, 89.157, 263.306, 90, 90, 90
R / Rfree (%)	22.8 / 26.3

The binding sites of Zinc atom in the Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4 (pdb code 8btl). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4, PDB code: 8btl:

Zinc binding site 1 out of 1 in the Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Complex Between the E2 Conjugating Enzyme UBE2A and the E3 Ligase Module From UBR4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn5201 b:63.8 occ:1.00 ND1 A:HIS4887 2.2 64.9 1.0 SG A:CYS4890 2.3 61.8 1.0 SG A:CYS4841 2.3 64.9 1.0 SG A:CYS4838 2.4 71.6 1.0 CB A:CYS4838 2.9 61.0 1.0 CG A:HIS4887 2.9 64.3 1.0 CB A:HIS4887 2.9 67.5 1.0 CB A:CYS4890 3.2 60.0 1.0 CE1 A:HIS4887 3.4 68.1 1.0 CB A:CYS4841 3.6 74.0 1.0 N A:HIS4887 3.6 64.0 1.0 CA A:HIS4887 3.9 67.3 1.0 N A:CYS4841 4.0 63.6 1.0 CD2 A:HIS4887 4.2 64.6 1.0 NE2 A:HIS4887 4.3 66.7 1.0 CA A:CYS4838 4.4 61.1 1.0 CA A:CYS4841 4.4 69.5 1.0 CA A:CYS4890 4.6 61.3 1.0 O A:GLU4843 4.7 73.8 1.0 CB A:ILE4840 4.8 60.0 1.0 N A:CYS4890 4.8 57.2 1.0 C A:VAL4886 4.9 63.9 1.0 C A:CYS4838 4.9 62.5 1.0 C A:HIS4887 4.9 70.3 1.0 O A:CYS4838 4.9 68.0 1.0 O A:HIS4887 5.0 72.4 1.0

S.Virdee, P.D.Mabbitt, L.Barnsby-Greer. An Atypical E3 Ligase Module in UBR4 Mediates Destabilization of N-Degron Substrates To Be Published.