Zinc in PDB 8ad1: Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State

All present enzymatic activity of Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State:
2.7.7.6;

The structure of Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State (pdb code 8ad1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State, PDB code: 8ad1:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:299.0 occ:1.00 CB D:CYS72 1.5 259.5 1.0 SG D:CYS88 2.3 248.5 1.0 SG D:CYS72 2.3 259.5 1.0 SG D:CYS85 2.3 253.6 1.0 CB D:CYS88 2.4 248.5 1.0 CA D:CYS72 2.6 259.5 1.0 N D:CYS72 2.8 259.5 1.0 N D:CYS88 3.0 248.5 1.0 CA D:CYS88 3.3 248.5 1.0 CE D:LYS87 3.5 243.7 1.0 NZ D:LYS74 3.6 240.4 1.0 CB D:CYS85 3.7 253.6 1.0 CD D:LYS87 3.7 243.7 1.0 CG D:LYS87 3.8 243.7 1.0 C D:CYS72 3.9 259.5 1.0 C D:LEU71 4.1 236.4 1.0 NZ D:LYS87 4.1 243.7 1.0 C D:LYS87 4.2 243.7 1.0 C D:CYS88 4.3 248.5 1.0 N D:GLY73 4.5 242.3 1.0 N D:LYS87 4.5 243.7 1.0 N D:GLY89 4.5 231.3 1.0 O D:CYS72 4.7 259.5 1.0 CA D:LYS87 4.7 243.7 1.0 O D:LEU71 4.8 236.4 1.0 CB D:LYS87 4.8 243.7 1.0 CB D:LEU71 4.9 236.4 1.0 CA D:LEU71 4.9 236.4 1.0 N D:LEU71 5.0 236.4 1.0 CE D:LYS74 5.0 240.4 1.0

Zinc binding site 2 out of 2 in the Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase at U-Rich Pause Bound to Rna Putl Triple Mutant - Pause Prone, Closed Clamp State within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1503 b:222.6 occ:1.00 CB D:CYS895 1.9 153.0 1.0 SG D:CYS814 2.3 165.8 1.0 SG D:CYS898 2.3 143.8 1.0 CB D:CYS814 2.3 165.8 1.0 SG D:CYS888 2.3 149.8 1.0 SG D:CYS895 2.3 153.0 1.0 CB D:CYS888 2.9 149.8 1.0 CA D:CYS895 3.4 153.0 1.0 CA D:CYS814 3.5 165.8 1.0 N D:CYS814 3.6 165.8 1.0 CB D:CYS898 3.6 143.8 1.0 CA D:CYS888 3.7 149.8 1.0 N D:CYS895 4.0 153.0 1.0 C D:CYS895 4.3 153.0 1.0 N D:CYS898 4.4 143.8 1.0 CA D:CYS898 4.5 143.8 1.0 C D:CYS814 4.6 165.8 1.0 CD D:ARG883 4.6 141.8 1.0 O D:CYS895 4.7 153.0 1.0 C D:CYS888 4.7 149.8 1.0 N D:ASP889 4.7 153.7 1.0 C D:ASP813 4.7 161.3 1.0 N D:CYS888 4.8 149.8 1.0 NE D:ARG883 4.9 141.8 1.0

S.Dey, C.Batisse, J.Shukla, M.W.Webster, M.Takacs, C.Saint-Andre, A.Weixlbaumer. Structural Insights Into Rna-Mediated Transcription Regulation in Bacteria. Mol.Cell V. 82 3885 2022.
ISSN: ISSN 1097-2765
PubMed: 36220101
DOI: 10.1016/J.MOLCEL.2022.09.020