Zinc in PDB 7zo5: L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product

All present enzymatic activity of L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product:
3.5.2.6;

The structure of L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product, PDB code: 7zo5 was solved by P.Hinchliffe, J.Spencer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.53 / 1.43
Space group	P 64 2 2
Cell size a, b, c (Å), α, β, γ (°)	105.61, 105.61, 98.45, 90, 90, 120
R / Rfree (%)	15.9 / 17.9

The binding sites of Zinc atom in the L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product (pdb code 7zo5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product, PDB code: 7zo5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:26.4 occ:1.00 NE2 A:HIS225 2.0 25.9 1.0 O A:HOH424 2.1 33.1 1.0 NE2 A:HIS89 2.1 25.3 1.0 OD2 A:ASP88 2.1 24.7 1.0 H9 A:JO9303 2.2 49.4 0.8 O1 A:JO9303 2.3 28.6 0.8 O1 A:JO9303 2.3 41.5 0.2 H9 A:JO9303 2.4 56.8 0.2 N A:JO9303 2.4 41.2 0.8 N A:JO9303 2.5 47.3 0.2 CG A:ASP88 3.0 27.2 1.0 CE1 A:HIS225 3.0 26.7 1.0 CE1 A:HIS89 3.0 24.5 1.0 C4 A:JO9303 3.0 34.9 0.8 CD2 A:HIS225 3.1 27.6 1.0 C4 A:JO9303 3.1 42.4 0.2 CD2 A:HIS89 3.1 23.8 1.0 HE1 A:HIS225 3.2 32.0 1.0 HE1 A:HIS89 3.2 29.4 1.0 HD2 A:HIS225 3.3 33.1 1.0 OD1 A:ASP88 3.3 22.9 1.0 C3 A:JO9303 3.3 39.5 0.8 HD2 A:HIS89 3.3 28.5 1.0 C3 A:JO9303 3.4 45.7 0.2 H10 A:JO9303 3.5 47.6 0.8 H10 A:JO9303 3.5 58.1 0.2 C5 A:JO9303 3.5 39.7 0.8 C5 A:JO9303 3.6 48.4 0.2 ZN A:ZN302 3.7 23.6 1.0 H2 A:JO9303 3.7 58.0 0.2 H2 A:JO9303 3.7 51.9 0.8 HE1 A:HIS84 3.8 30.0 1.0 HG A:SER185 3.9 35.3 1.0 H7 A:JO9303 4.0 47.3 0.8 H7 A:JO9303 4.1 54.8 0.2 ND1 A:HIS225 4.1 27.2 1.0 ND1 A:HIS89 4.1 23.4 1.0 CG A:HIS225 4.2 24.5 1.0 CG A:HIS89 4.2 25.4 1.0 HB2 A:ASP88 4.2 24.6 1.0 CB A:ASP88 4.3 20.5 1.0 O A:JO9303 4.3 30.4 0.8 O A:JO9303 4.3 41.0 0.2 C A:JO9303 4.3 43.2 0.8 C1 A:JO9303 4.3 40.7 0.8 CE1 A:HIS84 4.4 25.0 1.0 C A:JO9303 4.4 48.4 0.2 NE2 A:HIS84 4.4 24.1 1.0 C1 A:JO9303 4.4 47.7 0.2 N2 A:JO9303 4.4 42.2 0.8 H1 A:JO9303 4.4 51.9 0.8 H1 A:JO9303 4.4 58.0 0.2 C14 A:JO9303 4.4 42.3 0.8 C14 A:JO9303 4.5 49.2 0.2 N2 A:JO9303 4.5 49.8 0.2 H8 A:JO9303 4.6 36.5 0.8 O3 A:JO9303 4.6 48.3 0.2 C6 A:JO9303 4.6 44.3 0.8 H8 A:JO9303 4.6 49.2 0.2 O3 A:JO9303 4.6 39.9 0.8 H23 A:JO9303 4.6 50.6 0.8 OG A:SER185 4.6 29.4 1.0 C6 A:JO9303 4.7 50.6 0.2 H24 A:JO9303 4.8 50.8 0.8 HH2 A:TRP17 4.8 38.7 1.0 HB2 A:PRO224 4.8 31.3 1.0 HB3 A:ASP88 4.8 24.6 1.0 S A:JO9303 4.8 38.9 0.8 NE2 A:HIS160 4.8 21.4 1.0 HB2 A:HIS86 4.8 25.0 1.0 H23 A:JO9303 4.8 59.8 0.2 S A:JO9303 4.8 48.1 0.2 HZ3 A:TRP17 4.9 36.6 1.0 H24 A:JO9303 4.9 59.0 0.2 HD1 A:HIS89 4.9 28.0 1.0 HD1 A:HIS225 4.9 32.6 1.0 HD21 A:LEU38 5.0 33.0 1.0

Zinc binding site 2 out of 2 in the L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:23.6 occ:1.00 O A:HOH424 1.8 33.1 1.0 NE2 A:HIS160 2.0 21.4 1.0 ND1 A:HIS86 2.0 21.4 1.0 NE2 A:HIS84 2.0 24.1 1.0 CD2 A:HIS160 2.9 21.6 1.0 CE1 A:HIS84 3.0 25.0 1.0 CE1 A:HIS86 3.0 21.4 1.0 O3 A:JO9303 3.0 48.3 0.2 HB2 A:HIS86 3.0 25.0 1.0 CD2 A:HIS84 3.0 24.2 1.0 HD2 A:HIS160 3.0 25.9 1.0 O3 A:JO9303 3.0 39.9 0.8 CG A:HIS86 3.1 21.4 1.0 CE1 A:HIS160 3.1 21.7 1.0 HE1 A:HIS84 3.1 30.0 1.0 H9 A:JO9303 3.1 56.8 0.2 HE1 A:HIS86 3.1 25.7 1.0 H9 A:JO9303 3.2 49.4 0.8 H24 A:JO9303 3.2 50.8 0.8 HD2 A:HIS84 3.2 29.1 1.0 C14 A:JO9303 3.3 42.3 0.8 H24 A:JO9303 3.4 59.0 0.2 HE1 A:HIS160 3.4 26.0 1.0 C14 A:JO9303 3.4 49.2 0.2 HD2 A:HIS89 3.4 28.5 1.0 CB A:HIS86 3.5 20.9 1.0 O1 A:JO9303 3.5 41.5 0.2 O1 A:JO9303 3.5 28.6 0.8 HB3 A:HIS86 3.6 25.0 1.0 ZN A:ZN301 3.7 26.4 1.0 ND1 A:HIS84 4.0 26.1 1.0 N A:JO9303 4.1 47.3 0.2 N A:JO9303 4.1 41.2 0.8 CG A:HIS160 4.1 23.6 1.0 CD2 A:HIS89 4.1 23.8 1.0 CG A:HIS84 4.1 21.6 1.0 NE2 A:HIS86 4.1 22.6 1.0 C4 A:JO9303 4.1 34.9 0.8 C4 A:JO9303 4.1 42.4 0.2 ND1 A:HIS160 4.2 23.1 1.0 CD2 A:HIS86 4.2 21.7 1.0 OD1 A:ASP88 4.2 22.9 1.0 NE2 A:HIS89 4.3 25.3 1.0 H7 A:JO9303 4.3 47.3 0.8 N2 A:JO9303 4.3 42.2 0.8 H7 A:JO9303 4.4 54.8 0.2 HG A:SER185 4.4 35.3 1.0 HG23 A:THR161 4.4 30.5 1.0 N2 A:JO9303 4.4 49.8 0.2 C3 A:JO9303 4.5 39.5 0.8 C3 A:JO9303 4.5 45.7 0.2 HE2 A:PHE124 4.5 45.4 1.0 HB2 A:SER185 4.7 31.5 1.0 HD1 A:HIS84 4.8 31.3 1.0 H23 A:JO9303 4.8 50.6 0.8 H A:HIS86 4.8 26.2 1.0 HE2 A:HIS86 4.9 27.2 1.0 CA A:HIS86 4.9 21.2 1.0 OD2 A:ASP88 4.9 24.7 1.0 HD1 A:HIS160 5.0 27.8 1.0 CG A:ASP88 5.0 27.2 1.0 HG21 A:THR161 5.0 30.5 1.0 H23 A:JO9303 5.0 59.8 0.2

P.Hinchliffe, J.Spencer. L1 Metallo-Beta-Lactamase in Complex with A Mecillinam Degradation Product To Be Published.