Zinc in PDB 7wu0: Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3

All present enzymatic activity of Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3:
2.7.11.1;

The binding sites of Zinc atom in the Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3 (pdb code 7wu0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3, PDB code: 7wu0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3 within 5.0Å range: probe atom residue distance (Å) B Occ f:Zn200 b:65.8 occ:1.00 N f:LYS143 2.4 55.4 1.0 C f:LYS143 2.4 55.4 1.0 N f:CYS144 2.6 52.2 1.0 CA f:LYS143 2.7 55.4 1.0 C f:GLY142 2.7 57.2 1.0 N f:CYS145 2.8 57.1 1.0 O f:LYS143 2.9 55.4 1.0 O f:GLY142 3.2 57.2 1.0 SG f:CYS145 3.5 57.1 1.0 CA f:CYS144 3.5 52.2 1.0 CA f:GLY142 3.5 57.2 1.0 C f:CYS144 3.5 52.2 1.0 CA f:CYS145 3.7 57.1 1.0 O f:CYS141 3.7 56.8 1.0 N f:GLY142 4.0 57.2 1.0 C f:CYS141 4.1 56.8 1.0 CB f:LYS143 4.2 55.4 1.0 CB f:CYS145 4.2 57.1 1.0 O f:CYS144 4.7 52.2 1.0 O2' 2:A1291 4.7 82.0 1.0 OH f:TYR140 4.8 64.1 1.0 SG f:CYS141 4.8 56.8 1.0 CB f:CYS144 4.9 52.2 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of A Human Pre-40S Ribosomal Subunit - State RRP12- B3 within 5.0Å range: probe atom residue distance (Å) B Occ y:Zn500 b:42.9 occ:1.00 SG y:CYS273 2.3 21.6 1.0 SG y:CYS270 2.3 23.3 1.0 SG y:CYS288 2.3 19.2 1.0 SG y:CYS285 2.6 24.6 1.0 CB y:CYS270 3.3 23.3 1.0 CB y:CYS273 3.3 21.6 1.0 CB y:CYS288 3.6 19.2 1.0 CB y:CYS285 3.7 24.6 1.0 N y:CYS288 3.8 19.2 1.0 N y:CYS273 3.9 21.6 1.0 CG2 y:THR292 4.0 19.8 1.0 CA y:CYS288 4.2 19.2 1.0 CA y:CYS273 4.3 21.6 1.0 N y:GLY289 4.5 17.2 1.0 CB y:HIS287 4.6 21.5 1.0 C y:CYS288 4.6 19.2 1.0 CA y:CYS270 4.6 23.3 1.0 C y:HIS287 4.9 21.5 1.0 C y:GLY272 4.9 19.8 1.0 ND2 y:ASN290 4.9 17.1 1.0 N y:ASN290 5.0 17.1 1.0

J.Cheng, B.Lau, M.Thoms, M.Ameismeier, O.Berninghausen, E.Hurt, R.Beckmann. The Nucleoplasmic Phase of Pre-40S Formation Prior to Nuclear Export. Nucleic Acids Res. V. 50 11924 2022.
ISSN: ESSN 1362-4962
PubMed: 36321656
DOI: 10.1093/NAR/GKAC961