Zinc in PDB 7v8g: Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain

All present enzymatic activity of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain:
2.3.2.27; 2.3.2.31;

The structure of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain, PDB code: 7v8g was solved by J.Liu, Y.Wang, L.Pan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.36 / 2.75
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	136.896, 35.141, 145.871, 90, 93.69, 90
R / Rfree (%)	22 / 27.8

The binding sites of Zinc atom in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain (pdb code 7v8g). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain, PDB code: 7v8g:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn801 b:108.6 occ:1.00 SG D:CYS702 2.2 104.7 1.0 SG D:CYS725 2.2 100.5 1.0 SG D:CYS722 2.4 123.6 1.0 CB D:CYS702 2.4 84.7 1.0 SG D:CYS699 2.6 103.8 1.0 CB D:CYS722 3.4 116.8 1.0 CB D:CYS725 3.4 114.3 1.0 N D:CYS702 3.4 92.6 1.0 CA D:CYS702 3.5 89.9 1.0 CB D:CYS699 3.8 104.0 1.0 N D:CYS725 4.2 119.4 1.0 C D:CYS702 4.3 89.1 1.0 N D:CYS722 4.4 114.5 1.0 CA D:CYS725 4.4 120.6 1.0 CA D:CYS722 4.5 114.2 1.0 C D:VAL701 4.6 86.2 1.0 N D:GLY703 4.7 94.1 1.0 CB D:VAL701 4.9 87.1 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn802 b:151.5 occ:1.00 SG D:CYS717 2.2 158.8 1.0 SG D:CYS747 2.4 109.6 1.0 SG D:CYS744 2.5 107.1 1.0 SG D:CYS719 2.6 116.8 1.0 CB D:CYS719 3.1 121.7 1.0 CB D:CYS744 3.5 116.2 1.0 CB D:CYS747 3.7 102.7 1.0 CB D:CYS717 3.7 138.2 1.0 N D:CYS747 4.0 111.9 1.0 CA D:CYS719 4.3 124.0 1.0 CB D:ALA746 4.4 108.9 1.0 CD2 D:LEU714 4.4 135.0 1.0 N D:CYS719 4.4 132.1 1.0 CA D:CYS747 4.4 98.8 1.0 CA D:CYS717 4.7 138.6 1.0 C D:ALA746 4.9 110.8 1.0 CB D:LEU714 5.0 138.5 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn801 b:45.6 occ:1.00 SG C:CYS725 2.3 50.2 1.0 SG C:CYS702 2.3 45.4 1.0 SG C:CYS722 2.4 48.9 1.0 SG C:CYS699 2.4 40.0 1.0 CB C:CYS702 2.8 37.2 1.0 CB C:CYS699 3.3 49.5 1.0 CB C:CYS725 3.3 50.4 1.0 CB C:CYS722 3.4 47.3 1.0 N C:CYS702 3.5 45.7 1.0 CA C:CYS702 3.7 44.0 1.0 N C:CYS722 4.0 52.8 1.0 CA C:CYS722 4.2 56.3 1.0 N C:CYS725 4.2 49.1 1.0 C C:CYS702 4.4 48.6 1.0 CA C:CYS725 4.4 53.9 1.0 N C:GLY703 4.6 47.4 1.0 C C:VAL701 4.7 43.7 1.0 CA C:CYS699 4.7 52.1 1.0 O C:CYS722 4.7 48.7 1.0 C C:CYS722 4.8 56.3 1.0 CB C:VAL701 4.9 48.0 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Hoip RING1 Domain Bound to IPAH1.4 Lrr Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn802 b:76.5 occ:1.00 SG C:CYS747 2.3 78.8 1.0 SG C:CYS719 2.5 62.3 1.0 SG C:CYS744 2.6 60.8 1.0 SG C:CYS717 2.8 85.8 1.0 CB C:CYS719 3.1 60.8 1.0 CB C:CYS747 3.2 67.2 1.0 CB C:CYS717 3.2 80.9 1.0 CB C:CYS744 3.4 71.3 1.0 N C:CYS747 3.7 70.1 1.0 N C:CYS719 3.9 73.2 1.0 CA C:CYS747 4.0 68.0 1.0 CA C:CYS719 4.1 70.8 1.0 CB C:ALA746 4.2 61.8 1.0 N C:GLU718 4.3 77.9 1.0 CA C:CYS717 4.4 80.8 1.0 C C:ALA746 4.6 64.7 1.0 C C:CYS717 4.8 75.4 1.0 NH1 C:ARG749 4.8 79.0 1.0 CA C:ALA746 4.8 61.5 1.0 C C:CYS747 4.8 68.2 1.0 CA C:CYS744 4.9 67.5 1.0 O C:CYS719 4.9 74.8 1.0 C C:GLU718 5.0 69.3 1.0 N C:ALA746 5.0 65.3 1.0 C C:CYS719 5.0 66.5 1.0

J.Liu, Y.Wang, D.Wang, Y.Wang, X.Xu, Y.Zhang, Y.Li, M.Zhang, X.Gong, Y.Tang, L.Shen, M.Li, L.Pan. Mechanistic Insights Into the Subversion of the Linear Ubiquitin Chain Assembly Complex By the E3 Ligase IPAH1.4 of Shigella Flexneri. Proc.Natl.Acad.Sci.Usa V. 119 76119 2022.
ISSN: ESSN 1091-6490
PubMed: 35294289
DOI: 10.1073/PNAS.2116776119