Atomistry » Zinc » PDB 7ubj-7uhy » 7uhw
Atomistry »
  Zinc »
    PDB 7ubj-7uhy »
      7uhw »

Zinc in PDB 7uhw: Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K

Enzymatic activity of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K

All present enzymatic activity of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K:
1.1.1.1;

Protein crystallography data

The structure of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K, PDB code: 7uhw was solved by B.V.Plapp, L.Gakhar, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.92 / 1.30
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 44.37, 51.55, 92.57, 91.8, 103.02, 110.19
R / Rfree (%) 12.9 / 16.5

Other elements in 7uhw:

The structure of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K also contains other interesting chemical elements:

Fluorine (F) 10 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K (pdb code 7uhw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K, PDB code: 7uhw:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 7uhw

Go back to Zinc Binding Sites List in 7uhw
Zinc binding site 1 out of 4 in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn401

b:11.7
occ:1.00
 O1 A:PFB404 1.9 11.0 1.0
NE2 A:HIS67 2.0 11.1 1.0
SG A:CYS174 2.3 11.2 1.0
SG A:CYS46 2.4 11.6 1.0
C7 A:PFB404 2.9 13.1 1.0
CE1 A:HIS67 3.1 10.2 1.0
CD2 A:HIS67 3.1 11.0 1.0
CB A:CYS46 3.3 12.0 1.0
C5N A:NAJ403 3.4 9.9 1.0
CB A:CYS174 3.4 10.8 1.0
OG A:SER48 3.8 10.9 1.0
C6N A:NAJ403 4.0 9.3 1.0
C4N A:NAJ403 4.0 9.7 1.0
CB A:SER48 4.1 11.0 1.0
F6 A:PFB404 4.1 14.2 1.0
ND1 A:HIS67 4.2 11.4 1.0
C1 A:PFB404 4.2 13.2 1.0
CG A:HIS67 4.2 10.5 1.0
NH2 A:ARG369 4.6 12.4 1.0
C6 A:PFB404 4.6 15.2 1.0
CA A:CYS174 4.7 10.0 1.0
CA A:CYS46 4.8 10.0 1.0
OE2 A:GLU68 4.8 13.2 1.0
N A:SER48 4.9 10.0 1.0
N1N A:NAJ403 4.9 9.0 1.0
CE2 A:PHE93 5.0 12.3 1.0

Zinc binding site 2 out of 4 in 7uhw

Go back to Zinc Binding Sites List in 7uhw
Zinc binding site 2 out of 4 in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn402

b:12.0
occ:1.00
 SG A:CYS100 2.3 12.6 1.0
SG A:CYS111 2.3 11.5 1.0
SG A:CYS97 2.4 13.5 1.0
SG A:CYS103 2.4 12.2 1.0
CB A:CYS111 3.3 11.2 1.0
CB A:CYS103 3.4 11.1 1.0
CB A:CYS100 3.4 13.4 1.0
CB A:CYS97 3.4 14.0 1.0
N A:CYS97 3.6 11.9 1.0
CA A:CYS111 3.7 9.9 1.0
CA A:CYS97 3.9 12.9 1.0
N A:CYS100 3.9 14.1 1.0
N A:GLY98 4.0 13.8 1.0
N A:LEU112 4.0 10.5 1.0
N A:CYS103 4.2 11.6 1.0
CA A:CYS100 4.2 14.6 1.0
C A:CYS97 4.3 14.4 1.0
C A:CYS111 4.3 10.4 1.0
CA A:CYS103 4.3 11.1 1.0
N A:LYS99 4.4 15.7 1.0
C A:GLN96 4.6 11.9 1.0
N A:LYS113 4.9 11.3 1.0
C A:CYS100 4.9 14.3 1.0
O A:CYS100 4.9 13.2 1.0
CA A:GLN96 4.9 11.8 1.0
CG A:LYS113 4.9 14.6 1.0
O A:HOH880 5.0 29.8 1.0
CA A:GLY98 5.0 15.2 1.0

Zinc binding site 3 out of 4 in 7uhw

Go back to Zinc Binding Sites List in 7uhw
Zinc binding site 3 out of 4 in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn401

b:14.5
occ:1.00
 O1 B:PFB404 1.9 14.9 1.0
NE2 B:HIS67 2.0 13.2 1.0
SG B:CYS174 2.3 14.3 1.0
SG B:CYS46 2.3 14.7 1.0
C7 B:PFB404 3.0 16.0 1.0
CE1 B:HIS67 3.0 13.0 1.0
CD2 B:HIS67 3.1 13.4 1.0
CB B:CYS46 3.3 13.1 1.0
C5N B:NAJ403 3.4 12.6 1.0
CB B:CYS174 3.4 12.2 1.0
OG B:SER48 3.8 13.8 1.0
C4N B:NAJ403 4.0 13.3 1.0
C6N B:NAJ403 4.0 12.1 1.0
CB B:SER48 4.0 13.1 1.0
F6 B:PFB404 4.0 17.5 1.0
ND1 B:HIS67 4.2 13.9 1.0
CG B:HIS67 4.2 13.2 1.0
C1 B:PFB404 4.2 14.8 1.0
NH2 B:ARG369 4.6 17.2 1.0
C6 B:PFB404 4.6 15.5 1.0
CA B:CYS174 4.8 12.0 1.0
CA B:CYS46 4.8 14.7 1.0
OE2 B:GLU68 4.8 16.5 1.0
N B:SER48 4.9 13.2 1.0
CE2 B:PHE93 4.9 13.2 1.0
N1N B:NAJ403 5.0 12.4 1.0

Zinc binding site 4 out of 4 in 7uhw

Go back to Zinc Binding Sites List in 7uhw
Zinc binding site 4 out of 4 in the Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Horse Liver Alcohol Dehydrogenase G173A, Complexed with Nad+ and 2,3, 4,5,6-Pentafluorobenzyl Alcohol at 120 K within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn402

b:15.1
occ:1.00
 SG B:CYS111 2.3 15.1 1.0
SG B:CYS100 2.3 15.8 1.0
SG B:CYS97 2.3 18.6 1.0
SG B:CYS103 2.4 14.2 1.0
CB B:CYS111 3.3 13.4 1.0
CB B:CYS100 3.4 17.1 1.0
CB B:CYS103 3.4 14.5 1.0
CB B:CYS97 3.4 17.0 1.0
N B:CYS97 3.5 16.4 1.0
CA B:CYS111 3.7 11.8 1.0
N B:CYS100 3.9 16.8 1.0
CA B:CYS97 3.9 16.3 1.0
N B:GLY98 3.9 16.9 1.0
N B:LEU112 4.0 13.9 1.0
CA B:CYS100 4.2 18.4 1.0
N B:CYS103 4.2 13.7 1.0
C B:CYS111 4.3 12.6 1.0
C B:CYS97 4.3 16.8 1.0
CA B:CYS103 4.4 13.1 1.0
N B:LYS99 4.5 18.7 1.0
C B:GLN96 4.6 14.3 1.0
N B:LYS113 4.8 14.7 1.0
C B:CYS100 4.9 16.2 1.0
CA B:GLN96 4.9 13.8 1.0
CA B:GLY98 4.9 19.2 1.0
CG B:LYS113 5.0 20.3 1.0
O B:CYS100 5.0 15.9 1.0
O B:HOH821 5.0 31.5 1.0

Reference:

B.V.Plapp, L.Gakhar, R.Subramanian. Dependence of Crystallographic Atomic Displacement Factors on Temperature (25-150 K) For Complexes of Horse Liver Alcohol Dehydrogenases Acta Crystallogr.,Sect.D V. D78 1221 2022.
ISSN: ESSN 1399-0047
DOI: 10.1107/S2059798322008361
Page generated: Wed Oct 30 12:10:38 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy