Zinc in PDB 7ubn: Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex

All present enzymatic activity of Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex:
2.7.7.6;

The structure of Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex (pdb code 7ubn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex, PDB code: 7ubn:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1501 b:108.5 occ:1.00 SG D:CYS88 2.3 63.5 1.0 SG D:CYS70 2.3 61.3 1.0 SG D:CYS72 2.3 65.8 1.0 SG D:CYS85 2.3 64.0 1.0 CB D:CYS85 3.3 64.0 1.0 CB D:CYS70 3.3 61.3 1.0 CB D:CYS72 3.4 65.8 1.0 N D:CYS72 3.7 65.8 1.0 CB D:CYS88 4.0 63.5 1.0 CA D:CYS72 4.0 65.8 1.0 N D:CYS88 4.2 63.5 1.0 CB D:LYS74 4.3 67.3 1.0 N D:LYS74 4.4 67.3 1.0 C D:CYS72 4.4 65.8 1.0 N D:GLY73 4.4 62.9 1.0 N D:LEU71 4.4 63.0 1.0 CB D:LYS87 4.5 77.3 1.0 CA D:CYS70 4.6 61.3 1.0 CG D:LYS87 4.7 77.3 1.0 N D:LYS87 4.7 77.3 1.0 CA D:CYS85 4.7 64.0 1.0 CA D:CYS88 4.7 63.5 1.0 CD2 D:TYR75 4.7 61.9 1.0 C D:CYS70 4.8 61.3 1.0 C D:LYS87 4.8 77.3 1.0 C D:LEU71 4.9 63.0 1.0 CA D:LYS87 4.9 77.3 1.0 CA D:LYS74 5.0 67.3 1.0

Zinc binding site 2 out of 3 in the Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:155.6 occ:1.00 N D:THR816 2.2 102.7 1.0 C D:GLY815 2.4 102.9 1.0 C D:CYS814 2.4 92.3 1.0 N D:GLY815 2.5 102.9 1.0 SG D:CYS814 2.5 92.3 1.0 O D:CYS814 2.7 92.3 1.0 CB D:THR816 2.7 102.7 1.0 CB D:CYS814 2.8 92.3 1.0 CA D:THR816 2.9 102.7 1.0 CA D:GLY815 2.9 102.9 1.0 O D:GLY815 2.9 102.9 1.0 CA D:CYS814 3.1 92.3 1.0 CG2 D:THR816 3.4 102.7 1.0 CB D:ASP889 3.7 81.1 1.0 NH2 D:ARG883 3.8 80.3 1.0 OG1 D:THR816 3.9 102.7 1.0 N D:CYS814 4.0 92.3 1.0 C D:THR816 4.3 102.7 1.0 N D:ASP889 4.3 81.1 1.0 CA D:ASP889 4.6 81.1 1.0 O D:THR816 4.7 102.7 1.0 CG D:ASP889 4.8 81.1 1.0 OD2 D:ASP889 4.8 81.1 1.0

Zinc binding site 3 out of 3 in the Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Transcription Antitermination Complex: Nusa-Containing "Engaged" Qlambda-Loading Complex within 5.0Å range: probe atom residue distance (Å) B Occ Q:Zn1500 b:128.0 occ:1.00 SG Q:CYS121 2.3 93.3 1.0 SG Q:CYS147 2.3 106.5 1.0 SG Q:CYS144 2.3 112.2 1.0 SG Q:CYS118 2.3 90.4 1.0 CB Q:CYS147 2.6 106.5 1.0 CB Q:CYS121 3.0 93.3 1.0 CB Q:CYS118 3.1 90.4 1.0 CB Q:CYS144 3.1 112.2 1.0 N Q:CYS147 3.5 106.5 1.0 CA Q:CYS147 3.7 106.5 1.0 N Q:CYS121 3.7 93.3 1.0 CA Q:CYS121 4.0 93.3 1.0 CA Q:GLY125 4.3 94.4 1.0 O Q:CYS144 4.4 112.2 1.0 CA Q:GLY151 4.5 85.3 1.0 CA Q:CYS144 4.5 112.2 1.0 CA Q:CYS118 4.6 90.4 1.0 N Q:GLY125 4.6 94.4 1.0 C Q:CYS147 4.6 106.5 1.0 C Q:CYS144 4.7 112.2 1.0 C Q:ARG146 4.7 112.4 1.0 N Q:GLY151 4.9 85.3 1.0 C Q:CYS121 4.9 93.3 1.0 C Q:ASP120 4.9 106.9 1.0 C Q:GLY151 5.0 85.3 1.0 CB Q:ARG146 5.0 112.4 1.0

Z.Yin, J.G.Bird, J.T.Kaelber, B.E.Nickels, R.H.Ebright. In Transcription Antitermination By Q Lambda , Nusa Induces Refolding of Q Lambda to Form A Nozzle That Extends the Rna Polymerase Rna-Exit Channel. Proc.Natl.Acad.Sci.Usa V. 119 78119 2022.
ISSN: ESSN 1091-6490
PubMed: 35951650
DOI: 10.1073/PNAS.2205278119