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Zinc in PDB 7shq: Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin.

Enzymatic activity of Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin.

All present enzymatic activity of Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin.:
2.7.11.20;

Protein crystallography data

The structure of Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin., PDB code: 7shq was solved by A.Piserchio, E.A.Isiorho, D.Jeruzalmi, K.N.Dalby, R.Ghose, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.66 / 2.34
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 58.495, 58.495, 365.779, 90, 90, 120
R / Rfree (%) 22.9 / 25.2

Other elements in 7shq:

The structure of Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin. also contains other interesting chemical elements:

Calcium (Ca) 2 atoms
Magnesium (Mg) 5 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin. (pdb code 7shq). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin., PDB code: 7shq:

Zinc binding site 1 out of 1 in 7shq

Go back to Zinc Binding Sites List in 7shq
Zinc binding site 1 out of 1 in the Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A Functional Construct of Eukaryotic Elongation Factor 2 Kinase in Complex with Calmodulin. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn801

b:19.6
occ:1.00
ND1 A:HIS260 2.0 15.4 1.0
NE2 A:HIS312 2.0 13.0 1.0
SG A:CYS314 2.3 21.3 1.0
SG A:CYS318 2.3 22.5 1.0
CE1 A:HIS260 2.9 21.8 1.0
CD2 A:HIS312 3.0 22.6 1.0
CE1 A:HIS312 3.0 20.7 1.0
CG A:HIS260 3.1 14.7 1.0
CB A:CYS318 3.2 17.1 1.0
CB A:CYS314 3.4 17.8 1.0
CA A:CYS314 3.4 31.3 1.0
CB A:HIS260 3.5 9.4 1.0
NE2 A:HIS260 4.0 18.6 1.0
CD2 A:HIS260 4.1 12.3 1.0
ND1 A:HIS312 4.1 28.0 1.0
CG A:HIS312 4.2 18.1 1.0
N A:CYS314 4.2 26.6 1.0
O A:HOH944 4.3 24.0 1.0
C A:CYS314 4.6 31.3 1.0
CA A:CYS318 4.6 17.4 1.0
N A:ASN315 4.6 32.4 1.0
O A:HOH903 4.6 18.6 1.0
C A:ALA313 4.8 25.5 1.0
O A:ALA313 4.8 35.6 1.0
CD1 A:LEU323 4.8 32.1 1.0

Reference:

A.Piserchio, E.A.Isiorho, K.Long, A.L.Bohanon, E.A.Kumar, N.Will, D.Jeruzalmi, K.N.Dalby, R.Ghose. Structural Basis For the Calmodulin-Mediated Activation of Eukaryotic Elongation Factor 2 Kinase. Sci Adv V. 8 O2039 2022.
ISSN: ESSN 2375-2548
PubMed: 35857468
DOI: 10.1126/SCIADV.ABO2039
Page generated: Wed Oct 30 11:12:33 2024

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