Zinc in PDB 7py0: Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation)

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation):
2.7.7.6;

The structure of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation) (pdb code 7py0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation), PDB code: 7py0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1502 b:378.7 occ:1.00 CB D:CYS72 2.1 292.8 1.0 SG D:CYS72 2.2 292.8 1.0 N D:CYS72 2.2 292.8 1.0 SG D:CYS70 2.3 280.4 1.0 CA D:CYS72 2.6 292.8 1.0 CB D:CYS88 2.9 265.3 1.0 C D:LEU71 3.4 262.1 1.0 N D:LEU71 3.7 262.1 1.0 C D:CYS72 3.8 292.8 1.0 N D:CYS88 3.8 265.3 1.0 CA D:LEU71 3.9 262.1 1.0 CA D:CYS88 4.0 265.3 1.0 CB D:CYS70 4.0 280.4 1.0 N D:GLY73 4.1 265.1 1.0 SG D:CYS88 4.1 265.3 1.0 CB D:LEU71 4.2 262.1 1.0 SG D:CYS85 4.2 248.4 1.0 C D:CYS70 4.3 280.4 1.0 O D:LEU71 4.4 262.1 1.0 CB D:LYS87 4.5 254.8 1.0 C D:LYS87 4.6 254.8 1.0 CA D:CYS70 4.6 280.4 1.0 O D:CYS72 4.8 292.8 1.0 O D:CYS70 5.0 280.4 1.0

Zinc binding site 2 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of E.Coli Rna Polymerase Elongation Complex Bound to Nusg (Nusg-Ec in More-Swiveled Conformation) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1503 b:321.7 occ:1.00 SG D:CYS895 2.3 214.9 1.0 SG D:CYS898 2.3 219.8 1.0 SG D:CYS814 2.3 231.2 1.0 SG D:CYS888 2.3 221.1 1.0 CB D:CYS814 2.8 231.2 1.0 CB D:CYS888 3.0 221.1 1.0 NH2 D:ARG883 3.2 200.1 1.0 CB D:CYS898 3.3 219.8 1.0 CA D:CYS888 3.4 221.1 1.0 CB D:CYS895 3.7 214.9 1.0 N D:CYS814 3.8 231.2 1.0 CA D:CYS814 3.9 231.2 1.0 N D:ASP889 4.0 212.5 1.0 N D:CYS895 4.1 214.9 1.0 C D:CYS888 4.3 221.1 1.0 OG1 D:THR890 4.4 198.5 1.0 CZ D:ARG883 4.4 200.1 1.0 CA D:CYS895 4.4 214.9 1.0 CA D:CYS898 4.5 219.8 1.0 N D:CYS898 4.5 219.8 1.0 N D:CYS888 4.6 221.1 1.0 O D:CYS895 4.6 214.9 1.0 OG1 D:THR816 4.6 232.3 1.0 C D:CYS814 4.9 231.2 1.0 C D:CYS895 4.9 214.9 1.0 NE D:ARG883 4.9 200.1 1.0

C.Zhu, X.Guo, P.Dumas, M.Takacs, M.Abdelkareem, A.Vanden Broeck, C.Saint-Andre, G.Papai, C.Crucifix, J.Ortiz, A.Weixlbaumer. Transcription Factors Modulate Rna Polymerase Conformational Equilibrium. Nat Commun V. 13 1546 2022.
ISSN: ESSN 2041-1723
PubMed: 35318334
DOI: 10.1038/S41467-022-29148-0