Zinc in PDB 7pp4: Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb

All present enzymatic activity of Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb:
2.7.7.6;

The structure of Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb (pdb code 7pp4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb, PDB code: 7pp4:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb within 5.0Å range: probe atom residue distance (Å) B Occ d:Zn1501 b:67.3 occ:1.00 SG d:CYS891 2.3 41.5 1.0 SG d:CYS978 2.3 42.3 1.0 SG d:CYS968 2.3 46.7 1.0 SG d:CYS975 2.3 39.5 1.0 CB d:CYS978 3.2 42.3 1.0 CB d:CYS891 3.2 41.5 1.0 CB d:CYS968 3.3 46.7 1.0 CA d:CYS968 3.4 46.7 1.0 CB d:CYS975 3.4 39.5 1.0 N d:ALA969 3.8 45.1 1.0 NH1 d:ARG963 3.9 45.1 1.0 N d:CYS975 3.9 39.5 1.0 OG1 d:THR970 4.0 44.2 1.0 C d:CYS968 4.1 46.7 1.0 N d:CYS891 4.1 41.5 1.0 CA d:CYS975 4.1 39.5 1.0 CA d:CYS891 4.3 41.5 1.0 CA d:CYS978 4.3 42.3 1.0 N d:CYS978 4.4 42.3 1.0 O d:CYS975 4.5 39.5 1.0 N d:CYS968 4.6 46.7 1.0 CG2 d:THR893 4.6 46.8 1.0 C d:CYS975 4.7 39.5 1.0 N d:THR970 4.7 44.2 1.0 CA d:ALA969 5.0 45.1 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Mycobacterium Tuberculosis Rna Polymerase Holoenzyme Comprising Sigma Factor Sigb within 5.0Å range: probe atom residue distance (Å) B Occ d:Zn1502 b:139.8 occ:1.00 SG d:CYS60 2.2 112.4 1.0 SG d:CYS75 2.2 114.7 1.0 SG d:CYS78 2.2 112.8 1.0 SG d:CYS62 2.2 113.6 1.0 CB d:CYS75 3.0 114.7 1.0 CB d:CYS60 3.0 112.4 1.0 CB d:CYS78 3.4 112.8 1.0 N d:CYS62 3.5 113.6 1.0 CB d:CYS62 3.6 113.6 1.0 N d:CYS78 3.9 112.8 1.0 N d:TYR61 4.0 107.6 1.0 CA d:CYS62 4.0 113.6 1.0 CB d:LYS64 4.1 115.7 1.0 CA d:CYS60 4.2 112.4 1.0 CA d:CYS78 4.2 112.8 1.0 N d:LYS64 4.2 115.7 1.0 C d:CYS60 4.3 112.4 1.0 N d:GLY63 4.4 114.8 1.0 CA d:CYS75 4.5 114.7 1.0 C d:CYS62 4.5 113.6 1.0 CB d:ARG77 4.5 113.7 1.0 C d:TYR61 4.6 107.6 1.0 CG2 d:VAL80 4.7 102.7 1.0 CA d:LYS64 4.8 115.7 1.0 C d:ARG77 4.8 113.7 1.0 CA d:TYR61 4.8 107.6 1.0 CD2 d:TYR65 4.9 116.5 1.0 C d:CYS75 4.9 114.7 1.0 N d:TYR65 4.9 116.5 1.0

Z.Morichaud, S.Trapani, R.Vishwakarma, L.Chaloin, C.Lionne, J.Lai-Kee-Him, P.Bron, K.Brodolin. Structural Basis of the Mycobacterial Stress-Response Rna Polymerase Auto-Inhibition Via Oligomerization Biorxiv 2022.
DOI: 10.1101/2022.07.04.498644