Zinc in PDB 7ovo: Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine

All present enzymatic activity of Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine:
2.4.2.29;

The structure of Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine, PDB code: 7ovo was solved by M.Sebastiani, A.Heine, K.Reuter, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.74 / 2.10
Space group	P 43 2 2
Cell size a, b, c (Å), α, β, γ (°)	100.429, 100.429, 202.853, 90, 90, 90
R / Rfree (%)	18.9 / 23.4

The binding sites of Zinc atom in the Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine (pdb code 7ovo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine, PDB code: 7ovo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:52.6 occ:1.00 ND1 A:HIS382 2.2 41.6 1.0 SG A:CYS351 2.4 55.9 1.0 SG A:CYS356 2.4 49.2 1.0 SG A:CYS353 2.4 52.3 1.0 CE1 A:HIS382 3.2 48.9 1.0 CB A:CYS351 3.2 55.1 1.0 CB A:CYS356 3.2 52.0 1.0 CG A:HIS382 3.2 45.9 1.0 CB A:CYS353 3.5 51.3 1.0 CB A:HIS382 3.6 39.5 1.0 N A:CYS356 4.0 56.1 1.0 CA A:HIS382 4.0 44.3 1.0 CA A:CYS356 4.2 50.3 1.0 N A:CYS353 4.3 60.4 1.0 NE2 A:HIS382 4.3 47.1 1.0 CD2 A:HIS382 4.4 46.0 1.0 CA A:CYS353 4.4 60.9 1.0 CA A:CYS351 4.5 59.6 1.0 O A:HOH634 4.7 60.2 1.0 O A:HIS382 4.8 42.0 1.0 C A:CYS351 4.8 62.5 1.0 C A:HIS382 4.9 42.0 1.0 O A:CYS353 4.9 55.3 1.0 C A:CYS353 4.9 57.7 1.0 CB A:LEU347 5.0 40.5 1.0 CB A:VAL348 5.0 46.3 1.0

Zinc binding site 2 out of 2 in the Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Heterodimeric Murine Trna-Guanine Transglycosylase in Complex with Queuine within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn502 b:44.4 occ:1.00 ND1 C:HIS348 2.2 41.8 1.0 SG C:CYS322 2.2 44.2 1.0 SG C:CYS317 2.3 46.7 1.0 SG C:CYS319 2.3 47.1 1.0 CE1 C:HIS348 3.2 43.4 1.0 CG C:HIS348 3.2 39.0 1.0 CB C:CYS317 3.2 40.6 1.0 CB C:CYS322 3.2 49.5 1.0 CB C:CYS319 3.3 47.1 1.0 CB C:HIS348 3.5 40.4 1.0 N C:CYS322 3.8 45.9 1.0 CA C:HIS348 4.0 43.8 1.0 CA C:CYS322 4.1 50.0 1.0 N C:CYS319 4.2 51.7 1.0 CA C:CYS319 4.3 51.2 1.0 NE2 C:HIS348 4.3 41.3 1.0 CD2 C:HIS348 4.3 41.0 1.0 O C:HOH760 4.5 43.1 1.0 CA C:CYS317 4.5 45.4 1.0 O C:HIS348 4.6 44.1 1.0 O C:CYS319 4.6 55.3 1.0 C C:CYS317 4.7 52.2 1.0 CB C:THR321 4.7 43.2 1.0 C C:CYS319 4.7 52.1 1.0 O C:CYS317 4.7 56.5 1.0 C C:HIS348 4.8 45.0 1.0 C C:THR321 4.8 46.8 1.0

M.Sebastiani, A.Heine, K.Reuter. Heterodimeric Trna-Guanine Transglycosylase From Mouse To Be Published.