Zinc in PDB 7oez: Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine

The structure of Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine, PDB code: 7oez was solved by K.A.Watson, G.Baltulionis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	77.05 / 2.48
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	154.1, 154.1, 87.83, 90, 90, 120
R / Rfree (%)	15.7 / 21.5

The structure of Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine (pdb code 7oez). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine, PDB code: 7oez:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn406 b:46.9 occ:0.43 OD1 A:ASP195 1.9 41.0 1.0 OD1 A:ASP261 2.2 34.8 1.0 NE2 A:HIS176 2.3 29.7 1.0 OD2 A:ASP261 2.5 50.6 1.0 CG A:ASP261 2.7 43.7 1.0 N A:LEU414 2.8 51.9 0.8 CG A:ASP195 2.9 30.8 1.0 CA A:LEU414 3.0 53.9 0.8 CD2 A:HIS176 3.1 28.7 1.0 OXT A:LEU414 3.1 60.8 0.8 ZN A:ZN407 3.3 42.0 0.5 OD2 A:ASP195 3.3 48.2 1.0 CE1 A:HIS176 3.3 30.2 1.0 C A:LEU414 3.5 71.1 0.7 OE1 A:GLU233 3.9 44.1 1.0 OE2 A:GLU234 4.0 46.9 1.0 OE2 A:GLU233 4.1 50.1 1.0 CD A:GLU233 4.2 39.7 1.0 CB A:ASP261 4.2 40.0 1.0 CB A:ASP195 4.2 26.1 1.0 CD1 A:LEU414 4.3 47.9 0.9 CB A:ASP196 4.3 25.6 1.0 CG A:HIS176 4.3 37.1 1.0 ND1 A:HIS176 4.4 26.9 1.0 CB A:LEU414 4.4 62.0 0.8 CA A:ASP195 4.6 25.1 1.0 O A:LEU414 4.7 82.0 1.0 SD A:MET262 4.7 48.0 1.0 CD A:GLU234 4.8 37.4 1.0 C A:ASP195 4.9 30.1 1.0 OG A:SER315 4.9 38.6 1.0 CG A:LEU414 4.9 51.5 0.9 CG A:ASP196 4.9 30.7 1.0 CA A:ASP261 5.0 29.4 1.0

Zinc binding site 2 out of 2 in the Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Leucine Aminopeptidase A Mature Enzyme in A Complex with Leucine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn407 b:42.0 occ:0.54 OD2 A:ASP195 1.9 48.2 1.0 OE2 A:GLU234 2.2 46.9 1.0 NE2 A:HIS343 2.2 46.9 1.0 OE1 A:GLU234 2.6 47.4 1.0 CD A:GLU234 2.7 37.4 1.0 OXT A:LEU414 2.8 60.8 0.8 CG A:ASP195 2.9 30.8 1.0 CD2 A:HIS343 3.1 44.5 1.0 N A:LEU414 3.1 51.9 0.8 OD1 A:ASP195 3.2 41.0 1.0 CE1 A:HIS343 3.2 51.1 1.0 C A:LEU414 3.2 71.1 0.7 ZN A:ZN406 3.3 46.9 0.4 CA A:LEU414 3.7 53.9 0.8 O A:LEU414 3.8 82.0 1.0 O A:HOH569 3.9 29.2 1.0 O A:HOH557 3.9 46.5 0.7 OE1 A:GLU233 4.1 44.1 1.0 CB A:ASP195 4.2 26.1 1.0 CG A:HIS343 4.2 37.3 1.0 ND1 A:HIS343 4.2 43.9 1.0 CG A:GLU234 4.2 29.1 1.0 NE2 A:HIS176 4.3 29.7 1.0 CE1 A:HIS176 4.4 30.2 1.0 O A:HOH689 4.7 60.0 0.7 O A:HOH779 4.9 50.1 0.8

G.Baltulionis, M.Blight, A.Robin, D.Charalampopoulos, K.A.Watson. The Role of Propeptide-Mediated Autoinhibition and Intermolecular Chaperone in the Maturation of Cognate Catalytic Domain in Leucine Aminopeptidase. J.Struct.Biol. V. 213 07741 2021.
ISSN: ESSN 1095-8657
PubMed: 33989771
DOI: 10.1016/J.JSB.2021.107741