Zinc in PDB 7krn: Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc

All present enzymatic activity of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc:
2.7.7.48; 3.6.4.12; 3.6.4.13;

The structure of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Aluminium	(Al)	1 atom
Fluorine	(F)	3 atoms

The binding sites of Zinc atom in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc (pdb code 7krn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc, PDB code: 7krn:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:52.8 occ:1.00 ND1 A:HIS295 2.1 23.2 1.0 SG A:CYS310 2.3 25.0 1.0 SG A:CYS301 2.3 23.9 1.0 SG A:CYS306 2.3 20.4 1.0 CE1 A:HIS295 2.7 23.2 1.0 O A:CYS306 2.9 20.4 1.0 CG A:HIS295 3.4 23.2 1.0 CB A:CYS301 3.5 23.9 1.0 CB A:CYS310 3.6 25.0 1.0 CB A:CYS306 3.7 20.4 1.0 N A:HIS295 3.9 23.2 1.0 C A:CYS306 3.9 20.4 1.0 CB A:HIS295 4.0 23.2 1.0 NE2 A:HIS295 4.0 23.2 1.0 CA A:CYS301 4.0 23.9 1.0 CD2 A:HIS295 4.3 23.2 1.0 CA A:CYS306 4.3 20.4 1.0 CA A:HIS295 4.5 23.2 1.0 O A:HIS295 4.5 23.2 1.0 CA A:CYS310 4.8 25.0 1.0 N A:CYS310 4.8 25.0 1.0 C A:TYR294 4.9 27.4 1.0 CA A:TYR294 4.9 27.4 1.0 N A:CYS301 4.9 23.9 1.0

Zinc binding site 2 out of 5 in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1002 b:69.9 occ:1.00 ND1 A:HIS642 2.1 27.8 1.0 SG A:CYS487 2.3 28.3 1.0 SG A:CYS646 2.3 38.2 1.0 SG A:CYS645 2.3 42.0 1.0 CE1 A:HIS642 2.8 27.8 1.0 CG A:HIS642 3.2 27.8 1.0 CB A:CYS646 3.4 38.2 1.0 CB A:CYS487 3.5 28.3 1.0 CE1 A:PHE571 3.7 18.9 1.0 CB A:HIS642 3.7 27.8 1.0 N A:CYS487 3.8 28.3 1.0 CB A:CYS645 3.8 42.0 1.0 NE2 A:HIS642 4.0 27.8 1.0 N A:CYS646 4.0 38.2 1.0 C A:CYS645 4.1 42.0 1.0 CD1 A:PHE571 4.1 18.9 1.0 CA A:CYS487 4.2 28.3 1.0 CD2 A:HIS642 4.2 27.8 1.0 CA A:CYS646 4.2 38.2 1.0 O A:CYS645 4.4 42.0 1.0 CA A:CYS645 4.6 42.0 1.0 CZ A:PHE571 4.8 18.9 1.0 C A:GLY486 4.8 23.2 1.0 CA A:HIS642 4.9 27.8 1.0

Zinc binding site 3 out of 5 in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn701 b:89.2 occ:1.00 SG E:CYS8 2.3 56.8 1.0 SG E:CYS26 2.3 52.0 1.0 SG E:CYS29 2.3 60.3 1.0 SG E:CYS5 2.3 47.2 1.0 CB E:CYS26 2.8 52.0 1.0 CB E:CYS29 2.8 60.3 1.0 CB E:CYS5 3.3 47.2 1.0 N E:CYS26 3.7 52.0 1.0 CA E:CYS26 3.8 52.0 1.0 CB E:CYS8 3.8 56.8 1.0 CA E:CYS29 4.0 60.3 1.0 N E:CYS29 4.1 60.3 1.0 N E:CYS8 4.2 56.8 1.0 O E:VAL98 4.4 69.2 1.0 C E:CYS26 4.6 52.0 1.0 CA E:CYS8 4.6 56.8 1.0 CB E:LEU7 4.6 52.9 1.0 CA E:CYS5 4.8 47.2 1.0 N E:LEU7 4.9 52.9 1.0 C E:LEU7 4.9 52.9 1.0 C E:LEU25 4.9 43.0 1.0 CD2 E:LEU25 4.9 43.0 1.0 CA E:GLY99 4.9 73.4 1.0

Zinc binding site 4 out of 5 in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn702 b:135.9 occ:1.00 ND1 E:HIS75 2.1 81.9 1.0 SG E:CYS72 2.3 81.1 1.0 SG E:CYS55 2.3 83.8 1.0 SG E:CYS50 2.3 77.8 1.0 CE1 E:HIS75 2.7 81.9 1.0 CB E:CYS72 3.2 81.1 1.0 CG E:HIS75 3.2 81.9 1.0 CB E:CYS55 3.2 83.8 1.0 CB E:CYS50 3.5 77.8 1.0 N E:CYS72 3.8 81.1 1.0 CB E:HIS75 3.8 81.9 1.0 NE2 E:HIS75 3.8 81.9 1.0 CA E:CYS72 4.0 81.1 1.0 CD2 E:HIS75 4.1 81.9 1.0 N E:HIS75 4.2 81.9 1.0 CB E:ALA52 4.4 80.8 1.0 CA E:CYS55 4.7 83.8 1.0 CA E:HIS75 4.7 81.9 1.0 C E:CYS72 4.7 81.1 1.0 CB E:SER74 4.9 80.7 1.0 C E:SER74 4.9 80.7 1.0 CA E:CYS50 4.9 77.8 1.0 C E:TYR71 5.0 78.3 1.0 N E:SER74 5.0 80.7 1.0

Zinc binding site 5 out of 5 in the Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Structure of Sars-Cov-2 Backtracked Complex Bound to NSP13 Helicase - NSP13(1)-Btc within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn703 b:106.6 occ:1.00 NE2 E:HIS33 2.0 59.5 1.0 ND1 E:HIS39 2.0 67.2 1.0 SG E:CYS19 2.3 55.1 1.0 SG E:CYS16 2.3 48.6 1.0 CG E:HIS39 2.9 67.2 1.0 CE1 E:HIS33 3.0 59.5 1.0 CD2 E:HIS33 3.1 59.5 1.0 CE1 E:HIS39 3.1 67.2 1.0 CB E:HIS39 3.1 67.2 1.0 N E:CYS19 3.6 55.1 1.0 CB E:CYS16 3.6 48.6 1.0 CB E:CYS19 3.7 55.1 1.0 O E:CYS16 3.9 48.6 1.0 CD2 E:HIS39 4.1 67.2 1.0 CA E:CYS19 4.1 55.1 1.0 ND1 E:HIS33 4.1 59.5 1.0 NE2 E:HIS39 4.1 67.2 1.0 CG E:HIS33 4.2 59.5 1.0 CB E:ALA18 4.2 57.2 1.0 C E:ALA18 4.3 57.2 1.0 C E:CYS16 4.5 48.6 1.0 CA E:HIS39 4.6 67.2 1.0 CA E:ALA18 4.7 57.2 1.0 CA E:CYS16 4.7 48.6 1.0 N E:ALA18 4.7 57.2 1.0 CB E:ALA110 5.0 59.1 1.0

B.Malone, J.Chen, Q.Wang, E.C.Llewellyn, Y.J.Choi, P.D.B.Olinares, X.Cao, C.Hernandez, E.Eng, B.T.Chait, D.E.Shaw, R.Landick, S.A.Darst, E.A.Campbell. Structural Basis For Backtracking By the Sars-Cov-2 Replication-Transcription Complex Proc.Natl.Acad.Sci.Usa 2021.
ISSN: ESSN 1091-6490