Zinc in PDB 7jhe: Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography

The structure of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography, PDB code: 7jhe was solved by M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Ofinfectious Diseases (Csgid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.73 / 2.25
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	170.859, 170.859, 52.797, 90.00, 90.00, 120.00
R / Rfree (%)	22.3 / 24.9

The structure of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography (pdb code 7jhe). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography, PDB code: 7jhe:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4401 b:38.3 occ:1.00 NE2 B:HIS83 2.0 56.2 1.0 SG B:CYS90 2.3 47.1 1.0 SG B:CYS74 2.3 35.3 1.0 SG B:CYS77 2.3 36.5 1.0 CD2 B:HIS83 3.0 55.4 1.0 CE1 B:HIS83 3.0 54.0 1.0 CB B:CYS74 3.2 37.6 1.0 CB B:CYS90 3.3 59.2 1.0 CB B:CYS77 3.4 34.3 1.0 N B:CYS77 3.7 38.0 1.0 CA B:CYS77 4.1 34.6 1.0 ND1 B:HIS83 4.1 60.9 1.0 CG B:HIS83 4.1 61.1 1.0 CA B:CYS90 4.5 67.9 1.0 CA B:CYS74 4.6 36.9 1.0 CB B:TYR76 4.6 51.6 1.0 CB B:LYS93 4.7 43.5 1.0 C B:TYR76 4.8 41.8 1.0 N B:LYS93 4.8 41.6 1.0 O B:CYS74 4.9 36.1 1.0 C B:CYS74 5.0 35.8 1.0

Zinc binding site 2 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4402 b:92.2 occ:1.00 SG B:CYS120 2.3 78.3 1.0 SG B:CYS117 2.3 75.0 1.0 SG B:CYS128 2.3 74.8 1.0 SG B:CYS130 2.4 0.5 1.0 CB B:CYS117 3.3 76.2 1.0 O B:HOH4504 3.4 60.0 1.0 CB B:CYS130 3.4 0.7 1.0 CB B:CYS120 3.4 74.9 1.0 CB B:CYS128 3.5 87.5 1.0 N B:CYS120 3.7 73.6 1.0 CA B:CYS128 4.0 80.6 1.0 N B:CYS130 4.1 0.9 1.0 CA B:CYS120 4.2 77.0 1.0 CA B:CYS130 4.4 0.8 1.0 C B:CYS128 4.4 79.2 1.0 N B:SER129 4.4 89.4 1.0 CB B:VAL119 4.5 76.5 1.0 CA B:CYS117 4.7 68.8 1.0 C B:VAL119 4.8 73.3 1.0 CE B:LYS124 4.9 0.4 1.0 C B:CYS120 5.0 74.0 1.0 N B:GLY121 5.0 72.2 1.0

M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Of Infectious Diseases(Csgid). Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with 2'-O-Methylated M7GPPPA Cap-1 and Sah Determined By Fixed-Target Serial Crystallography To Be Published.