Zinc in PDB 6uau: Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose

The structure of Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose, PDB code: 6uau was solved by P.S.Vieira, L.Cabral, P.A.C.R.Costa, C.R.Santos, M.T.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.97 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	38.991, 79.054, 46.960, 90.00, 101.77, 90.00
R / Rfree (%)	19.8 / 23.2

The binding sites of Zinc atom in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose (pdb code 6uau). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose, PDB code: 6uau:

Zinc binding site 1 out of 1 in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E102A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaritriose and Laminaribiose within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn306 b:39.5 occ:1.00 OD2 A:ASP118 2.1 21.4 1.0 OE2 A:GLU114 2.3 24.9 1.0 O A:HOH610 2.5 24.7 1.0 CG A:ASP118 2.9 21.3 1.0 OD1 A:ASP118 3.1 21.1 1.0 CD A:GLU114 3.2 24.2 1.0 CG A:GLU114 3.3 24.1 1.0 O A:HOH410 4.1 31.0 1.0 CB A:ASP118 4.3 21.1 1.0 OE1 A:GLU114 4.3 24.4 1.0 NH2 A:ARG156 4.4 22.3 1.0 O A:GLU114 4.6 22.0 1.0 CB A:GLU114 4.8 23.8 1.0

C.R.Santos, E.A.Lima, F.Mandelli, M.T.Murakami. Structural Insights Into Beta-1,3-Glucan Cleavage By A Glycoside Hydrolase Family Nat.Chem.Biol. 2020.
ISSN: ESSN 1552-4469
DOI: 10.1038/S41589-020-0554-5