Atomistry » Zinc » PDB 5b25-5bqk » 5b2q
Atomistry »
  Zinc »
    PDB 5b25-5bqk »
      5b2q »

Zinc in PDB 5b2q: Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam)

Protein crystallography data

The structure of Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam), PDB code: 5b2q was solved by H.Hirano, H.Nishimasu, T.Nakane, R.Ishitani, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.29 / 1.70
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 81.562, 158.977, 96.738, 90.00, 106.88, 90.00
R / Rfree (%) 18.3 / 20.6

Other elements in 5b2q:

The structure of Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam) also contains other interesting chemical elements:

Calcium (Ca) 17 atoms
Chlorine (Cl) 2 atoms
Sodium (Na) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam) (pdb code 5b2q). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam), PDB code: 5b2q:

Zinc binding site 1 out of 1 in 5b2q

Go back to Zinc Binding Sites List in 5b2q
Zinc binding site 1 out of 1 in the Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Francisella Novicida CAS9 Rha in Complex with Sgrna and Target Dna (Tgg Pam) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1701

b:26.0
occ:1.00
SG A:CYS657 2.3 27.1 1.0
SG A:CYS814 2.3 24.6 1.0
SG A:CYS460 2.3 27.1 1.0
SG A:CYS817 2.4 27.4 1.0
CB A:CYS460 3.2 25.9 1.0
CB A:CYS657 3.2 26.1 1.0
CB A:CYS817 3.2 29.6 1.0
CB A:CYS814 3.4 25.2 1.0
N A:CYS814 3.8 23.9 1.0
NH2 A:ARG536 4.1 28.5 1.0
N A:CYS817 4.1 27.4 1.0
CA A:CYS814 4.2 23.7 1.0
OG A:SER462 4.2 28.6 1.0
CA A:CYS817 4.3 27.7 1.0
CB A:SER462 4.4 23.7 1.0
CA A:CYS460 4.6 25.9 1.0
CA A:CYS657 4.6 25.7 1.0
O A:HOH2181 4.7 32.2 1.0
C A:CYS814 4.8 26.4 1.0
NH1 A:ARG528 4.8 36.0 1.0
O A:CYS814 4.9 30.9 1.0
C A:THR813 5.0 29.0 1.0

Reference:

H.Hirano, J.S.Gootenberg, T.Horii, O.O.Abudayyeh, M.Kimura, P.D.Hsu, T.Nakane, R.Ishitani, I.Hatada, F.Zhang, H.Nishimasu, O.Nureki. Structure and Engineering of Francisella Novicida CAS9 Cell V. 164 950 2016.
ISSN: ISSN 1097-4172
PubMed: 26875867
DOI: 10.1016/J.CELL.2016.01.039
Page generated: Wed Dec 16 06:04:29 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy