Zinc in PDB 4ojy: 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus

All present enzymatic activity of 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus:
3.2.1.23;

The structure of 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus, PDB code: 4ojy was solved by H.V.Solomon, O.Tabachnikov, H.Feinberg, Y.Shoham, G.Shoham, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.43 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.730, 181.310, 197.660, 90.00, 90.00, 90.00
R / Rfree (%)	14.1 / 21.5

The binding sites of Zinc atom in the 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus (pdb code 4ojy). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus, PDB code: 4ojy:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:27.9 occ:1.00 SG A:CYS164 2.3 18.8 1.0 SG A:CYS169 2.4 24.0 1.0 SG A:CYS166 2.4 25.1 1.0 SG A:CYS124 2.4 27.8 1.0 CB A:CYS164 3.1 18.5 1.0 CB A:CYS166 3.4 19.3 1.0 CB A:CYS169 3.4 20.6 1.0 CB A:CYS124 3.5 25.0 1.0 N A:CYS166 3.8 20.2 1.0 O A:HOH816 3.9 22.8 1.0 N A:CYS169 4.1 21.9 1.0 CA A:CYS166 4.1 17.9 1.0 CA A:CYS124 4.2 27.1 1.0 CA A:CYS164 4.3 24.0 1.0 CA A:CYS169 4.3 19.9 1.0 C A:CYS164 4.4 22.1 1.0 CB A:THR126 4.6 25.9 1.0 N A:HIS165 4.6 18.9 1.0 O A:CYS166 4.7 22.9 1.0 OG1 A:THR126 4.7 23.9 1.0 C A:CYS166 4.7 19.9 1.0 O A:CYS164 4.8 24.0 1.0 C A:CYS124 4.8 32.4 1.0 N A:THR126 4.8 21.9 1.0 N A:PHE125 4.9 27.6 1.0 C A:HIS165 4.9 19.7 1.0 CB A:LEU168 4.9 18.8 1.0

Zinc binding site 2 out of 3 in the 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:23.2 occ:1.00 SG B:CYS164 2.3 19.6 1.0 SG B:CYS169 2.3 18.5 1.0 SG B:CYS124 2.4 17.1 1.0 SG B:CYS166 2.4 17.9 1.0 CB B:CYS164 3.1 16.2 1.0 CB B:CYS124 3.3 17.0 1.0 CB B:CYS166 3.4 13.9 1.0 CB B:CYS169 3.5 17.2 1.0 N B:CYS166 3.8 19.9 1.0 N B:CYS169 4.1 17.3 1.0 CA B:CYS166 4.1 15.1 1.0 CA B:CYS124 4.1 17.1 1.0 O B:HOH830 4.1 20.0 1.0 CA B:CYS164 4.3 20.6 1.0 CA B:CYS169 4.4 21.8 1.0 C B:CYS164 4.4 21.4 1.0 OG1 B:THR126 4.5 21.8 1.0 CB B:THR126 4.5 22.4 1.0 N B:HIS165 4.6 19.9 1.0 C B:CYS166 4.6 20.8 1.0 O B:CYS166 4.7 22.4 1.0 C B:CYS124 4.7 21.1 1.0 N B:THR126 4.7 16.2 1.0 O B:CYS164 4.8 23.1 1.0 CB B:LEU168 4.8 27.2 1.0 N B:PHE125 4.9 20.8 1.0 CE1 B:PHE125 4.9 26.5 1.0 C B:HIS165 4.9 22.1 1.0

Zinc binding site 3 out of 3 in the 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of 3D Structure of the E323A Catalytic Mutant of GAN42B, A GH42 Beta- Galactosidase From G. Stearothermophilus within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn701 b:25.0 occ:1.00 SG C:CYS164 2.3 21.0 1.0 SG C:CYS169 2.4 23.2 1.0 SG C:CYS166 2.4 23.1 1.0 SG C:CYS124 2.5 23.9 1.0 CB C:CYS164 3.2 23.4 1.0 CB C:CYS124 3.3 20.8 1.0 CB C:CYS166 3.4 20.1 1.0 CB C:CYS169 3.5 21.6 1.0 N C:CYS166 3.9 22.0 1.0 CA C:CYS124 4.1 20.7 1.0 N C:CYS169 4.1 16.8 1.0 CA C:CYS166 4.2 19.2 1.0 O C:HOH816 4.2 14.9 1.0 CA C:CYS169 4.4 22.7 1.0 CA C:CYS164 4.4 25.1 1.0 C C:CYS164 4.4 24.2 1.0 N C:HIS165 4.6 25.2 1.0 CB C:THR126 4.6 20.5 1.0 C C:CYS166 4.7 19.1 1.0 O C:CYS166 4.7 21.5 1.0 C C:CYS124 4.7 24.3 1.0 CB C:LEU168 4.7 24.0 1.0 O C:CYS164 4.7 17.7 1.0 N C:THR126 4.8 19.6 1.0 OG1 C:THR126 4.8 24.6 1.0 N C:PHE125 4.8 19.2 1.0

H.V.Solomon, O.Tabachnikov, H.Feinberg, L.Govada, N.E.Chayen, Y.Shoham, G.Shoham. Crystallization and Preliminary Crystallographic Analysis of Ganb, A GH42 Intracellular Beta-Galactosidase From Geobacillus Stearothermophilus. Acta Crystallogr.,Sect.F V. 69 1114 2013.
ISSN: ISSN 1744-3091
PubMed: 24100561
DOI: 10.1107/S1744309113023609