Zinc in PDB 1m2n: SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex

The structure of SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex, PDB code: 1m2n was solved by J.Chang, Y.Cho, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.91 / 2.60
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	84.720, 84.720, 193.087, 90.00, 90.00, 120.00
R / Rfree (%)	22.8 / 30.8

The binding sites of Zinc atom in the SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex (pdb code 1m2n). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex, PDB code: 1m2n:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn999 b:86.0 occ:1.00 SG A:CYS127 2.3 84.0 1.0 SG A:CYS124 2.3 84.7 1.0 SG A:CYS148 2.3 85.4 1.0 SG A:CYS145 2.3 84.3 1.0 CB A:CYS124 3.1 79.8 1.0 CB A:CYS127 3.2 83.1 1.0 N A:CYS148 3.5 85.2 1.0 CB A:CYS148 3.6 84.8 1.0 N A:CYS127 3.7 78.9 1.0 CB A:CYS145 3.7 85.6 1.0 CA A:CYS127 4.0 81.2 1.0 CB A:LYS147 4.0 85.2 1.0 CA A:CYS148 4.0 85.1 1.0 N A:GLY149 4.2 83.2 1.0 N A:SER150 4.3 80.8 1.0 C A:CYS148 4.4 84.5 1.0 CB A:SER150 4.5 80.4 1.0 CB A:ASN129 4.5 74.7 1.0 C A:LYS147 4.6 85.1 1.0 CA A:CYS124 4.6 76.5 1.0 C A:CYS127 4.6 81.9 1.0 CA A:LYS147 4.7 85.3 1.0 C A:SER126 4.8 77.2 1.0 O A:SER150 4.8 78.5 1.0 O A:CYS127 4.8 82.8 1.0 N A:SER126 4.9 73.3 1.0 ND2 A:ASN129 4.9 71.4 1.0 CB A:SER126 4.9 76.1 1.0 CA A:SER150 4.9 79.7 1.0 OG A:SER150 4.9 80.9 1.0 N A:LYS147 4.9 85.8 1.0 CA A:CYS145 5.0 86.5 1.0

Zinc binding site 2 out of 2 in the SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of SIR2 Homologues (D102G/F159A/R170A) Mutant-2'-O-Acetyl Adp Ribose Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn999 b:93.5 occ:1.00 SG B:CYS127 2.3 91.7 1.0 SG B:CYS145 2.3 92.0 1.0 SG B:CYS124 2.3 92.4 1.0 SG B:CYS148 2.3 94.3 1.0 CB B:CYS127 3.1 90.7 1.0 CB B:CYS145 3.2 91.6 1.0 CB B:CYS124 3.3 87.9 1.0 N B:CYS127 3.4 88.7 1.0 OG B:SER150 3.5 87.5 1.0 CB B:CYS148 3.7 95.0 1.0 CA B:CYS127 3.8 89.3 1.0 N B:CYS148 4.1 95.5 1.0 CA B:CYS148 4.4 95.1 1.0 CB B:SER126 4.5 88.6 1.0 CB B:ASN129 4.5 85.4 1.0 C B:CYS127 4.5 89.0 1.0 C B:SER126 4.6 88.9 1.0 N B:GLY149 4.6 94.2 1.0 CA B:CYS145 4.7 91.3 1.0 CB B:LYS147 4.7 94.2 1.0 CA B:CYS124 4.7 84.2 1.0 C B:CYS148 4.8 94.7 1.0 CA B:SER126 4.9 89.0 1.0 CB B:SER150 4.9 87.6 1.0 N B:ASN128 4.9 88.5 1.0 N B:SER126 4.9 88.6 1.0 N B:ASN129 4.9 87.0 1.0

J.H.Chang, H.C.Kim, K.Y.Hwang, J.W.Lee, S.P.Jackson, S.D.Bell, Y.Cho. Structural Basis For the Nad-Dependent Deacetylase Mechanism of SIR2 J.Biol.Chem. V. 277 34489 2003.
ISSN: ISSN 0021-9258
PubMed: 12091395
DOI: 10.1074/JBC.M205460200